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Inhibitory effect of heat shock protein 70 combined with bacillus calmette-guerin on mouse hepatoma Hcaf cells
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 17
- DOI:
- 10.3969/j.issn.1009-3079.2008.17.005
- Key Word:
- 热休克蛋白70;卡介苗;Hcaf肿瘤细胞;免疫组化
Abstract： 目的:观察联合应用热休克蛋白70(HSP70)与卡介苗(BCG)对Hcaf肿瘤的抑制作用,为应用肿瘤来源的HSP70治疗人类恶性肿瘤提供参考依据.方法:实验小鼠64只随机分为对照组、BCG免疫组、HSP70免疫组和联合组4组,每组16只.小鼠接种Hcaf细胞制成肿瘤模型.BCG组接种1mg BCG;HSP70组注射10μg HSP70,联合组以HSP70 10μg和BCG 1mg代替,对照组注射50μL PBS液.观察小鼠的生存时间、生存率和肿瘤大小等指标的变化.取肿瘤组织进行免疫组化实验,观察肿瘤细胞性状及AFP的表达情况.结果:应用免疫治疗后,小鼠肿瘤生长均得到不同程度的抑制,生存期均有延长,其中HSP70组和联合组与对照组相比差异显著(77.1±15.1d,82.8±13.2d vs 22.9±3.50d,均P<0.01),同时生存率也有所提高(41.7%,66.7% vs 0,both P<0.01).对照组及BCG免疫组AFP表达明显,而HSP70组和联合组AFP表达均受到不同程度抑制,其中联合组尤其显著.与对照组及BCG免疫组比较,HSP70组和联合组肿瘤细胞异型性均有所降低.结论:HSP70与BCG联合应用对Hcaf细胞有明显抑制作用,其疗效要优于单独应用HSP70或BCG.
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