Immunostimulatory effects of CpG-ODN 2216 on the activation of peripheral blood B lymphocytes and Th1-type cytokines from patients with hepatitis B virus infection
- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 14
- DOI:
- 10.3969/j.issn.1009-3079.2008.14.016
- Key Word:
- CpG-ODN 2216;抗原提呈细胞;B淋巴细胞;酶联免疫吸附试验;流式细胞术
Abstract： 目的:观察CpG-ODN 2216对乙型肝炎病毒(hepatitis B virus,HBV)感染者外周血单个核细胞(peripheral blood mononuclear cells,PBMC)中B淋巴细胞抗原提呈相关分子CD80、CD86、MHC-Ⅰ和MHC-Ⅱ分子表达的影响,同时观察CpG对Th1型细胞因子分泌的影响.方法:取HBV感染者PBMC与CpG-ODN 2216共培养48 h,用流式细胞技术检测B淋巴细胞表面CD80、CD86、MHC-Ⅰ和MHC-Ⅱ分子的表达,并与未加CpG组进行比较,同时检测培养液中Th1型细胞因子白介素-12(IL-12)和干扰素-γ(IFN-γ)的水平.结果: 经CpG-ODN 2216活化刺激后,PBMC中B淋巴细胞抗原提呈相关分子CD80、CD86、MHC-Ⅰ及MHC-Ⅱ分子的表达显著高于对照组(CD80: 48.84%±21.29% vs 28.57%±18.7%;CD86: 50.12%±19.70% vs 13.15%±8.81%;MHC-Ⅰ:2108.88±289.04 vs 1679.22±388.22;MHC-Ⅱ:1602.77±362.61 vs 941.88±237.35;均P<0.05);并且培养液中IFN-γ及IL-12的水平也显著高于对照组(IFN-γ:61.38±38.81 ng/L vs 47.35±38.76 ng/L;IL-12:7.80±4.34 ng/L vs 5.56±3.56 ng/L;均P<0.05).结论:CpG-ODN 2216能够提高HBV感染者B淋巴细胞表面抗原提呈相关分子和共刺激分子的表达,同时提高PBMC分泌Th1型细胞因子的能力.
- [1]司方明,娄海山,郑鹏远,等.树突状细胞治疗HBeAg阴性慢性乙型肝炎患者的临床观察[J].2007,(35).
- [2]Rou-Bing Li,Hong-Song Chen,Yao Xie,etc.Dendritic cells from chronic hepatitis B patients can induce HBV antigen-specific T cell responses[J].2004,(11).
- [3].病毒性肝炎防治方案[J].2001,(1).
- [4]Becker Y.CpG ODNs treatments of HIV-1 infected patients may cause the decline of transmission in high risk populations - a review, hypothesis and implications.[J].2005,30(2).
- [5]Roh S,Kim K.Overcoming tolerance in hepatitis B virus transgenic mice: a possible involvement of regulatory T cells.[J].2003,47(6).
- [6]Uematsu S,Akira S,Levy R,etc.Lymphoma immunotherapy with CpG oligodeoxynucleotides requires TLR9 either in the host or in the tumor itself.[J].2007,179(4).
- [7]HYUN-DONG CHANG,ANDREAS RADBRUCH.The Pro- and Anti-Inflammatory Potential of Interleukin-12[J].2007,1109(aug).
- [8]Daniel, Rodríguez-Pinto.B cells as antigen presenting cells.[J].2005,238(2).
- [9]Hilliard B,Murphy S,Tani M,etc.Astrocytes as antigen-presenting cells: expression of IL-12/IL-23.[J].2005,95(2).
- [10]Xiao JZ,Miyaji K,Iwabuchi N,etc.In vitro Th1 cytokine-independent th2 suppressive effects of bifidobacteria.[J].2007,51(7).
- [11]Vollmer J,Jurk M.Therapeutic applications of synthetic CpG oligodeoxynucleotides as TLR9 agonists for immune modulation.[J].2007,21(6).
- [12]Warren TL,Krieg AM,Dahle CE,etc.APC stimulated by CpG oligodeoxynucleotide enhance activation of MHC class I-restricted T cells.[J].2000,165(11).
- [13]Krieg AM,Ashman RF,Yi AK,etc.CpG DNA rescues B cells from apoptosis by activating NFkappaB and preventing mitochondrial membrane potential disruption via a chloroquine-sensitive pathway.[J].1999,11(12).