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Relationship between serum expression of MUC1,MUC2 and MUC5AC and gastric cancer
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 12
- DOI:
- 10.3969/j.issn.1009-3079.2008.12.008
- Key Word:
- 黏蛋白;胃癌;蛋白芯片
Abstract： 目的:探讨胃癌血清中MUC1、MUC2及MUC5AC与胃癌的关系.方法:利用黏蛋白MUC1、MUC2及MUC5AC的单抗及多抗,制备黏蛋白芯片,用免疫荧光原理进行检测,并用CEA作为指示指标,对30例胃癌患者及30例健康人进行黏蛋白血清水平的检测.结果:胃癌组与对照组相比,3种黏蛋白血清水平均增高,两组间差异显著.MUC1的阳性表达与胃癌TNM分期相关(P=0.0047),MUC2和MUC5AC的阳性表达与TNM分期无关(P=0.136.P=0.201).3种黏蛋白的表达均随胃癌分化程度降低而阳性表达增高的趋势,各单一黏蛋白以及黏蛋白两两之间联合用于胃癌诊断的敏感性及特异性均较高,其中单一黏蛋白中,MUC1的的敏感性和特异性最好,分别达到81.5%、75.8%,联合诊断(3种黏蛋白中至少2种阳性)的敏感性和特异性达到96.0%、82.9%.结论:检测血清黏蛋白MUC1、MUC2及MUC5AC水平,可以提高胃癌诊断的敏感性及特异性,为早期诊断提供新的思路,并对判断分期及预后提供帮助.
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