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Detection of the drug-resistant gene of Helicobacter pylori

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Author:: No author available

Journal Title:: WORLD CHINESE JOURNAL OF DIGESTOLOGY

Issue:: 5

DOI:: 10.3969/j.issn.1009-3079.2008.05.011

Key Word:: 幽门螺杆菌;耐药机制;耐药基因;检测方法

Abstract：抗生素的广泛应用使幽门螺杆菌(H pylor1)对抗生素的耐药性和耐药率逐渐增加,目前对幽门螺杆菌耐药的检测方法重点在对克拉霉素和甲硝唑两种抗生素耐药的检测.其检测方法主要是基于聚合酶链式反应为基础的分子生物学技术,其对克拉霉素发展前景广阔的是聚合酶链式反应.限制性片段长度多态性分析技术和实时定量聚合酶链式反应结合解链曲线分析技术,可对活检组织和粪便两种样本进行检测,同时可以对多种抗生素进行耐药检测:其对甲硝唑耐药的检测主要是免疫印迹法和聚合酶链式反应.限制性片段长度多态性分析技术,有望发展成检测H pylori耐药的常规项目.

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