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Expression of P27 and cyclin D1 and E expression in gastric cancer

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Author:: No author available

Journal Title:: WORLD CHINESE JOURNAL OF DIGESTOLOGY

Issue:: 36

DOI:: 10.3969/j.issn.1009-3079.2007.36.007

Key Word:: 胃癌;P27;周期蛋白D1;周期蛋白E

Abstract：目的:检测胃癌组织中P27的表达及其与cyclinD1、cyclin E表达的相关性,并探讨其意义.方法:临床病理资料齐全的胃癌蜡块标本54例,正常胃黏膜标本15例,采用SP免疫组化方法检测P27、cyclin D和cyclin E在其中的表达.结果:胃癌组织54例中有20例P27表达阳性(37.0%),正常胃组织中15例有11例P27呈阳性表达(73.3%),胃癌组织与正常胃组织相比,P27的表达有明显差异(P＜0.05),胃癌组织中P27的表达与患者的性别、年龄及肿瘤大小,浸润深度,分化程度无相关性(P＞0.05),而与TNM分期及有无淋巴结转移明显相关(P＜0.05),P27的表达与cyclin D1的表达呈负相关(r=-0.332),而与cyclin E的表达无明显相关性(P＞0.05).结论:胃癌组织中P27表达与正常胃组织有显著差异,胃癌组织中P27蛋白表达与淋巴结转移及临床分期密切相关,与cyclin E的表达呈负相关.

Reference

[1]葛建荣,任国平,姚航平.细胞周期素D1和p27kip1在肾癌中的表达及其意义[J].2007,(5).
[2]N, Kawamata,R, Morosetti,C W, Miller,etc.Molecular analysis of the cyclin-dependent kinase inhibitor gene p27/Kip1 in human malignancies.[J].1995,55.
[3]Khaled A,Fernandez-Valle C,Iacovelli J,etc.Serum and forskolin cooperate to promote G1 progression in Schwann cells by differentially regulating cyclin D1, cyclin E1, and p27(Kip) expression[J].2007,55(16).
[4]K, Polyak,M H, Lee,H, Erdjument-Bromage,etc.Cloning of p27Kip1, a cyclin-dependent kinase inhibitor and a potential mediator of extracellular antimitogenic signals.[J].1994,78(1).
[5]Jaromir Vlach,Silke Hennecke,Bruno Amati.Phosphorylation-dependent degradation of the cyclin-dependent kinase inhibitor p27[J].1997,16(17).
[6]O, Halevy,B G, Novitch,D B, Spicer,etc.Correlation of terminal cell cycle arrest of skeletal muscle with induction of p21 by MyoD.[J].1995,267.
[7]J, Worm,J, Bartkova,A F, Kirkin,etc.Aberrant p27Kip1 promoter methylation in malignant melanoma.[J].2000,19(44).
[8]Medema RH,Dijkers PF,Burgering BM,etc.Forkhead transcription factor FKHR-L1 modulates cytokine-dependent transcriptional regulation of p27(KIP1).[J].2000,20(24).
[9]Geisen C,Moroy T.The oncogenic activity of cyclin E is not confined to Cdk2 activation alone but relies on several other, distinct functions of the protein.[J].2002,277(42).
[10]H, Toyoshima,T, Hunter.p27, a novel inhibitor of G1 cyclin-Cdk protein kinase activity, is related to p21.[J].1994,78(1).
[11]P, Tan,B, Cady,M, Wanner,etc.The cell cycle inhibitor p27 is an independent prognostic marker in small (T1a,b) invasive breast carcinomas.[J].1997,57.
[12]M Pagano,SW Tam,AM Theodoras,etc.Role of the ubiquitin-proteasome pathway in regulating abundance of the cyclin-dependent kinase inhibitor p27[J].1995,269 (5224).
[13]K, Polyak,J Y, Kato,M J, Solomon,etc.p27Kip1, a cyclin-Cdk inhibitor, links transforming growth factor-beta and contact inhibition to cell cycle arrest.[J].1994,8.
[14]Dorsman JC,Nowee ME,Piek JM,etc.HER-2/neu and p27(Kip1) in progression of Fallopian tube carcinoma: an immunohistochemical and array comparative genomic hybridization study.[J].2007,51(5).
[15]Besson A,Clurman BE,Hwang HC,etc.Discovery of an oncogenic activity in p27Kip1 that causes stem cell expansion and a multiple tumor phenotype.[J].2007,21(14).
[16]Loda M,Bhattacharaya N,Lechpammer M,etc.Flavopiridol reduces malignant transformation of the esophageal mucosa in p27 knockout mice.[J].2005,24(10).
[17]J A, Pietenpol,S K, Bohlander,Y, Sato,etc.Assignment of the human p27Kip1 gene to 12p13 and its analysis in leukemias.[J].1995,55.
[18]C, Cianga,P, Cianga,L, Cozma,etc.Low p27(Kip1)expression is associated with poor prognostic factors in breast carcinomas.[J].1900,7(2).
[19]Turowska O,Master A,Tanski Z,etc.Elevated cyclin E level in human clear cell renal cell carcinoma: possible causes and consequences[J].2007,54(3).
[20]X, Qian,L, Jin,J P, Grande,etc.Transforming growth factor-beta and p27 expression in pituitary cells.[J].1996,137.
[21]S P, Singh,J, Lipman,H, Goldman,etc.Loss or altered subcellular localization of p27 in Barrett's associated adenocarcinoma.[J].1998,58.
[22]Yang W,Suldan Z,Pianetti S,etc.Repression of transcription of the p27(Kip1) cyclin-dependent kinase inhibitor gene by c-Myc.[J].2001,20(14).
[23]Ban H,Takasu S,Tsukamoto T,etc.Cyclin D1 overexpression in N-methyl-N'-nitro-N-nitrosoguanidine-induced rat gastric adenocarcinomas.[J].2007,59(3).
[24]Chen J,Willingham T,Shuford M,etc.Tumor suppression and inhibition of aneuploid cell accumulation in human brain tumor cells by ectopic overexpression of the cyclin-dependent kinase inhibitor p27KIP1.[J].1996.
[25]Andrew Koff,Fred Cross,Alfred Fisher,etc.Human cyclin E, a new cyclin that interacts with two members of the CDC2 gene family[J].1991,66(6).