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Expression of heparanase in tissue microarrays of primary hepatocellular carcinoma and its significance
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 33
- DOI:
- 10.3969/j.issn.1009-3079.2007.33.017
- Key Word:
- 乙酰肝素酶;癌;肝细胞;免疫组织化学;组织芯片
Abstract： 目的:探讨乙酰肝素酶(heparanase,HPA)蛋白在原发性肝细胞癌(HCC)组织芯片中的过度表达及临床意义.方法:125例HCC患者肝组织、48例肝癌患者癌旁组织、62例肝硬化患者肝组织及23例肝血管瘤患者相应正常肝组织构建组织微阵列.应用免疫组织化学检测HPA蛋白的表达水平,并分析其与HCC临床病理特征的关系.结果:HCC组织中的HPA蛋白的阳性率45.83%明显高于癌旁组织27.08%(χ2=2.23,P＜0.05),肝硬化6.45%(χ2=5.262,P＜0.05)和正常肝组织4.35%(χ2=3.895,P＜0.05).癌旁组织中的HPA蛋白阳性率明显高于肝硬化(χ2=2.882,P＜0.05)及正常肝组织(χ2=2.361,P＜0.05);HCC中临床TNM分期ⅠⅡ期HPA阳性率明显低于ⅢⅣ期(29.41% vs 67.31%,χ2=4.111,P＜0.05);HCC中无转移组HPA阳性率明显低于转移组(14.71% vs 63.33%,χ2=3.978,P＜0.05);HPA表达率在AFP≥400 μg/L和AFP＜400 μg/L组(52.05% vs 36.17%,χ2=2.071,P＜0.05)、有无门脉癌栓组(71.74% vs 29.73%,x2=4.472,P＜0.05)、多个和单个肿瘤结节组(73.91% vs 28.38%,χ2=4.847,P＜0.05)以及肿瘤直径≥5 cm和＜5 cm组(57.89% vs 25%,χ2=3.471,P＜0.01)分别具有显著性意义.HPA表达与年龄、性别、分化程度、有无肝硬化及肿瘤包膜浸润无关.结论:HPA高表达在HCC的发生、发展及转移中起重要作用.检测HPA蛋白指标有助于HCC诊断和判断患者预后.
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