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Distribution of pancreatic trypsin in pigs with chronic pancreatitis,and a positive trypsin standard for immunohistochemical studies

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Author:: No author available

Journal Title:: WORLD CHINESE JOURNAL OF DIGESTOLOGY

Issue:: 29

DOI:: 10.3969/j.issn.1009-3079.2007.29.007

Key Word:: 胰蛋白酶;慢性胰腺炎;纤维化;免疫组化

Abstract：目的:建立慢性胰腺炎(CP)胰蛋白酶免疫组化阳性判断标准,探讨胰蛋白酶在CP中的分布特征及与CP严重程度的关系.方法:取正常猪4例及不同程度CP猪14例的胰腺组织,用链菌素亲生物素-过氧化酶连接法(streptavidin peroxidase conjugated method,SP)免疫组化法检测胰蛋白酶在实验性CP胰腺组织中的分布特征并初步建立阳性判断标准,分析胰蛋白酶的阳性标准与CP严重程度的相关性.结果:胰蛋白酶在CP胰腺组织主要表现为腺泡细胞质内、间质及胰管内棕色或棕黄色染色,染色程度在轻度CP以强阳性(++)为主,中度CP以中等阳性(+)为主,重度CP以弱阳性(±)为主,3组染色程度之间差异有统计学意义(P＜0.05).胰蛋白酶强阳性染色与慢性胰腺炎严重程度具有相关性(r=0.742).结论:胰蛋白酶原异常活化可见于腺泡细胞质内、间质及胰管内,建立了胰蛋白酶阳性判断标准.随着慢性胰腺炎实质纤维化,胰蛋白酶阳性率降低.

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