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Detection of human carcinoma-embryonic antigen using fluorescent amplification catalyzed by T7 polymerase technique
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 27
- DOI:
- 10.3969/j.issn.1009-3079.2007.27.014
- Key Word:
- T7 RNA聚合酶;荧光扩增技术;癌胚抗原;酶联接免疫吸附剂测定
Abstract： 目的:建立检测人癌胚抗原(CEA)的T7 RNA聚合酶催化的荧光扩增技术(fluorescent amplification catalyzed by T7 polymerase technique,FACTT).方法:以亲和素作为连接分子,连接生物素化的检测抗体和生物素化的DNA,加入T7RNA聚合酶进行转录扩增反应,对生成的RNA产物进行荧光检测,并同时进行夹心酶联接免疫吸附剂测定(ELISA)方法检测人CEA.结果:成功的建立了检测人CEA的T7 RNA聚合酶催化的荧光扩增技术,其检测CEA的灵敏度达2×10-3 mg/L,比夹心ELISA方法灵敏度(0.25 mg/L)高125倍.结论:T7 RNA聚合酶催化的荧光扩增技术较夹心ELISA方法具有更高的敏感性,有可能作为一种新的检测方法用于临床的早期诊断.
- [1]P, Gold,S O, Freedman.Specific carcinoembryonic antigens of the human digestive system.[J].1965,122(3).
- [2]L J, Jones,S T, Yue,C Y, Cheung,etc.RNA quantitation by fluorescence-based solution assay: RiboGreen reagent characterization.[J].1998,265(2).
- [3]S, Hammarstrom,J E, Shively,R J, Paxton,etc.Antigenic sites in carcinoembryonic antigen.[J].1989,49.
- [4]T, Sano,C L, Smith,C R, Cantor.Immuno-PCR: very sensitive antigen detection by means of specific antibody-DNA conjugates.[J].1992,258(5079).
- [5]Yong-Chao Guo,Ya-Feng Zhou,Yan-Mei Qiao,etc.Phage display mediated immuno-PCR[J].2006,34(8).
- [6]Cheng X,Greene MI,Zhang H,etc.A sensitive and high-throughput assay to detect low-abundance proteins in serum.[J].2006,12(4).
- [7]E T, Butler,M J, Chamberlin.Bacteriophage SP6-specific RNA polymerase. I. Isolation and characterization of the enzyme.[J].1982,257(10).
- [8]D.A.Melton,P.A.Krieg,M.R.Rebagliati,etc.Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter[J].1984,12(18).
- [9]Allen RC,Rogelj S,Cordova SE,etc.An immuno-PCR method for detecting Bacillus thuringiensis Cry1Ac toxin.[J].2006,308(1).
- [10]Tian P,Mandrell R.Detection of norovirus capsid proteins in faecal and food samples by a real time immuno-PCR method[J].2006,100(3).
- [11]P, GOLD,S O, FREEDMAN.Demonstration of Tumor-Specific Antigens in Human Colonic Carcinomata by Immunological Tolerance and Absorption Techniques.[J].1965,121.