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Effects of an extract from Scutellaria barbatae D.DON on tumor angiogenesis in vitro
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 20
- DOI:
- 10.3969/j.issn.1009-3079.2007.20.008
- Key Word:
- 半枝莲;黄酮类化合物;血管生成;人脐静脉内皮细胞;人宫颈癌Hela细胞;血管内皮细胞生长因子;一氧化氮;酶联免疫吸附测定法
Abstract： 目的:探讨中药半枝莲黄酮类化合物A06对体外肿瘤血管生成的影响.方法:分别采用小管形成实验、Transwell小室、酶联免疫吸附测定法(ELISA)、硝酸还原酶法检测半枝莲黄酮类化合物A06对人脐静脉内皮细胞(HUVEC)小管形成、迁移及人宫颈癌Hela细胞血管内皮细胞生长因子(VEGF)、一氧化氮(NO)表达的影响.结果:与对照组相比,半枝莲黄酮类化合物A06(50和100 mg/L)都能有效抑制HUVEC细胞的迁移(37.7%±10.7%,13.7%±6.0% vs 68.0%±8.2%,均P＜0.01),减少小管样结构形成(9.7%±4.5%,1.8%±1.2% vs 30.2%±5.4%,均P＜0.05),以及降低24 h后Hela细胞VEGF和NO的表达(VEGF:135.6±14.6 ng/L,108.3±7.7 ng/L vs 231.1±6.4 ng/L,均P＜0.01;NO:42.3±2.3μmol/L,25.9±5.2 μmol/L vs 70.1±8.1 μmol/L,均P＜0.01).结论:半枝莲黄酮类化合物A06有抑制体外肿瘤血管生成的作用,可能与抑制肿瘤细胞血管生成相关因子的表达,抑制内皮细胞迁移、形成管样结构有关.
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