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Effects of histone deacetylase inhibitor on colon cancer cell growth and ID4 gene expression
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 18
- DOI:
- 10.3969/j.issn.1009-3079.2007.18.009
- Key Word:
- 组蛋白去乙酰酶抑制剂;结肠癌;ID4基因
Abstract： 目的:研究组蛋白去乙酰酶抑制剂曲古菌素A(TSA)对结肠癌细胞Caco2增殖的影响和机制.方法:5-500 μg/LTSA处理结肠癌Caco2细胞0-5 d,在药物处理前及36 h后用MTT法检测细胞增殖状况.流式细胞仪检测细胞周期的改变.RT-PCR检测ID4 mRNA的表达.结果:TSA在100 μg/L浓度以上可以明显抑制结肠癌Caco2细胞的增殖(P＜0.05),但在5和20μg/L浓度时抑制作用不明显.100 μg/L TSA可导致细胞G1期阻滞,但没有诱导明显的细胞凋亡.RT-PCR显示20 μg/LTSA作用36 h后ID4mRNA表达增强(O＜0.05),在100 μg/L其表达显著增强(P＜0.01).结论:TSA可以通过阻滞Caco2细胞的G1期和重新表达ID4来发挥抑癌作用.
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