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Effects of fragile histidine triad gene transduction on growth of Hep3B human hepatocellular carcinoma cells

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Author:
No author available
Journal Title:
WORLD CHINESE JOURNAL OF DIGESTOLOGY
Issue:
18
DOI:
10.3969/j.issn.1009-3079.2007.18.005
Key Word:
肝癌;基因治疗;脆性组氨酸三联体

Abstract: 目的:研究外源性人脆性组氨酸三联体(FHIT)基因的高表达对人肝癌细胞Hep3B生长的影响.方法:构建一个包含FHIT基因的真核表达载体pcDNA3.1(+)/FHIT,体外转染人肝癌细胞Hep3B,逆转录-聚合酶链反应(RT-PCR),蛋白印迹(Western blot)分别检测目的基因不同水平的表达,通过细胞生长试验、流式细胞仪分析技术检测细胞转染前后的细胞增殖、细胞周期和细胞凋亡的变化.结果:转染pcDNA3.1(+)/FHIT后Hep3B细胞的FHIT-mRNA,FHIT蛋白表达阳性,转染后对Hep3B细胞的生长有抑制作用,早期细胞凋亡增加,与对照组比较差异有显著性(72.23±0.84 vx 54.36±0.78,53.17±0.52,P>0.05).结论:FHIT基因通过诱导肿瘤细胞发生凋亡并导致其发生G1期阻滞,在肿瘤基因治疗方面发挥作用.

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