Effects of gastric electrical stimulation on hippocampus gastric distension responsive neurons and the expression of motilin and neuronal nitric oxide synthase in rats
- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 13
- DOI:
- 10.3969/j.issn.1009-3079.2007.13.002
- Key Word:
- 胃电刺激;海马;胃动素;一氧化氮合酶;免疫荧光组织化学;放射免疫;大鼠
Abstract： 目的:观察胃电刺激(GES)对大鼠海马CA1区胃扩张(GD)相关神经元放电活动的影响及脑内一氧化氮合酶(nNOS)和胃动素(MTL)表达的改变,初步探讨GES的中枢作用机制.方法:选用成年Wistar大鼠58只,采用细胞外记录神经元单位放电方法,记录海马CA1区神经元自发放电活动,根据神经元对胃扩张刺激反应的不同,分为胃扩张兴奋性神经元(GD-E)和胃扩张抑制性神经元(GD-I).观察3组不同参数的胃电刺激(GES-A,GES-B和GES-C)对CA1区内GD-E和GD-I放电频率的影响:GES-A(6 mA,0.3 ms,40 Hz,2 s-on,3s-off)为标准参数;GES-B的串刺激波宽增至3ms;GES-C的串刺激频率减少至20 Hz,其他参数均同GES-A.采用放射免疫法检测胃电刺激对大鼠不同脑区MTL含量的影响;并采用免疫荧光组织化学染色方法观察胃电刺激2 h对大鼠海马内nNOS免疫阳性神经元的表达.结果:CA1区记录的87个神经元中有79个神经元(90.8%)对胃扩张刺激(GD,3-5 mL,10-30 s)有反应,其中40个(50.6%)为GD-E申经元,39个(49.4%)为GD-I神经元.GES-A,-B,-C分别兴奋了62.5%,100%和62.3%的GD-E神经元,GES-B对GD-E神经元的作用明显强于GES-C(神经元兴奋率GES-B 100% vs GES-C 62.3%,P＜0.05).在GD-I神经元中有63.6%,85.7%和50%的GD-I神经元分别被GES-A,-B和-C所兴奋,其中GES-C的兴奋作用较弱(P=0.041,vs GES-A;P=0.021,vs GES-B).GES-A刺激胃窦部2 h,下丘脑(48.93±6.98 fmol/mg vs 96.23±12.93 fmol/mg,P＜0.01)、中脑(30.96±4.86fmol/mg vs 53.17±8.96 fmol/mg,P＜0.05)、延脑(46.27±7.83 fmol/mg vs 73.86±9.37 fmol/mg,P＜0.05)和海马(32.23±6.51 fmol/mgvs 62.72±10.07 fmol/mg,P＜0.05)中MTL免疫反应物(MTL-IR)的含量与假手术对照组相比明显减少,但脑桥内MTL-IR的含量与假手术对照组相比无显著差异;且海马CA1区(16.75±0.91 cells/mm2 vs 20.46±1.30 cells/mm2.P＜0.05)和CA2-3区(14.91±1.17 cells/mm2 vs 18.73±1.10 cells/mm2,P＜0.05)nNOS免疫阳性神经元表达明显减少.结论:GES可兴奋海马CA1区内胃扩张反应性神经元,电刺激作用的强弱与GES刺激的频率和单个电刺激的持续时间有关;脑内MTL和海马内nNOS可能参与了GES的中枢作用机制.
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