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Growth suppression of human hepatic carcinoma cell by induction of human oncostatin M gene via recombinant adenovirus
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 7
- DOI:
- 10.3969/j.issn.1009-3079.2007.07.014
- Key Word:
- 腺病毒载体;抑瘤素M;基因转染;逆转录聚合酶链反应;肝癌
Abstract： 目的:构建含人抑瘤素M(human oncostatin M,HOSM)基因的复制缺陷型重组腺病毒载体AD-HOSM,观察其对人肝癌细胞株HepG2在体外生长抑制情况.方法:通过同源重组方法构建含HOSM基因的缺陷型重组腺病毒载体AD-HOSM,用报告基因AD-GFP检测腺病毒的对人肝癌细胞系的转染效率:人肝癌细胞株HepG2转染含HOSM基因的缺陷型腺病毒载体AD-HOSM后,用RT-PCR法检测外源HOSM基因在其中的表达;台盼蓝染色、细胞计数法检测AD-HOSM对HepG2的体外增殖抑制情况.结果:成功构建了重组腺病毒载体AD-HOSM,AD-HOSM对肝癌细胞HepG2有较高的转染效率,并且转染效率与病毒的剂量呈正相关.AD-HOSM感染HepG2细胞48h,HOSM基因在转染细胞能有效的表达.体外试验示,AD-HOSM转染的细胞的增殖却受到明显的抑制.结论:重组腺病毒介导HOSM表达能有效抑制HepG2在体外的增殖,提示重组腺病毒介导的HOSM基因治疗可能成为肝癌治疗的候选方案.
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