You Position: Home > Paper
Protective effects of carbachol on intestinal epithelial cells in rats with gut ischemia-reperfusion
( views:173, downloads:0 )
- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 6
- DOI:
- 10.3969/j.issn.1009-3079.2007.06.016
- Key Word:
- 缺血/再灌流;卡巴胆碱;肠上皮细胞;大鼠
Abstract： 目的:研究缺血/再灌流(I/R)时卡巴胆碱(Ca)对肠上皮细胞的保护作用及可能机制.方法:阻断Wistar大鼠SMA血量建立I/R模型,并以肠袋内注射卡巴胆碱进行处理.采用病理学方法观察不同时间肠上皮细胞损伤指数,免疫组化法检测肠上皮细胞TNF-α表达的变化;生化法检测肠组织中DAO含量(nkat/gpro)的变化.结果:I/R+Ca组再灌流各时间点肠上皮细胞病理变化较I/R+NS组轻(P＜0.01);I/R+Ca组再灌流各时间点肠袋组织DAO含量较I/R+NS组显著增加(0 min:42.01±7.17 vs 18.50±7.83;30 min:44.51±8.00 vs 20.00±5.83:60 min:35.67±7.00 vs 16.34±8.17:120 min:39.00±7.33 vs 21.84±6.67:240 min:53.34±8.17 vs 45.68±6.00;均P＜0.01),而I/R+Ca组再灌流各时间点肠上皮细胞中呈TNF-α阳性细胞数较I/R+NS组明显减少(0 min:204.4±12 vs 246.4±15.6;30 min:198.4±11.2 vs 230.4±14.4;60 min:234.4±12.1 vs 270.4±17.1;120 min:225.4±10.2 vs 260.4±18.5;240 min:196.4±10.8 vs 220.4±1 8.2;均P＜0.01).结论:卡巴胆碱能抑制缺血-再灌流时肠上皮细胞中TNF-α表达,减轻病理损害,对肠上皮细胞具有保护作用.
- [1]吕艺,姜小国,曹卫红,等.卡巴胆碱对肠缺血-再灌流动物脏器功能的保护作用[J].2006,(3).
- [2]黎君友,吕艺,付小兵,等.二胺氧化酶在创伤后肠道损伤中变化及意义[J].2000,(8).
- [3]盛新华,石汉平.肠道在多器官功能障碍综合征中的作用[J].2005,(16).
- [4]李晓芳.严重烧伤后胃肠道功能障碍的机制和防治[J].2006,(9).
- [5]姜小国,石德光,胡森,等.迷走神经及其递质乙酰胆碱的抗炎作用研究进展[J].2003,(1).
- [6]张喜平,张宇.急性胰腺炎肠道屏障损害机制的研究进展[J].2006,(4).
- [7]胡森,曹卫红,孙丹,等.卡巴胆碱对肠部分缺血-再灌注损伤所致全身炎症反应和多器官功能障碍的影响[J].2005,(1).
- [8]Newman WalterH,Castresana ManuelR,Detmer Kristina,etc.An IkappaB-alpha mutant inhibits cytokine gene expression and proliferation in human vascular smooth muscle cells.[J].2002,102(2).
- [9]L V, Borovikova,S, Ivanova,M, Zhang,etc.Vagus nerve stimulation attenuates the systemic inflammatory response to endotoxin.[J].2000,405(6785).
- [10]Mihály Boros,Shigeko Takaichi,Kaoru Hatanaka.Ischemic Time-Dependent Microvascular Changes and Reperfusion Injury in the Rat Small Intestine[J].1995,59(2).
- [11]Mole DJ,Taylor MA,McFerran NV,etc.The isolated perfused liver response to a 'second hit' of portal endotoxin during severe acute pancreatitis.[J].2005,5(4).
- [12]J J, van Lanschot,K, Mealy,D W, Wilmore.The effects of tumor necrosis factor on intestinal structure and metabolism.[J].1990,212(6).
- [13]Schmid-Schonbein GW,Mitsuoka H.Mechanisms for blockade of in vivo activator production in the ischemic intestine and multi-organ failure.[J].2000,14(5).
- [14]Daniel Cloa,Emma Folch-Puy.Oxygen Free Radicals and the Systemic Inflammatory Response[J].2004,56(4).