Expression of ezrin, E-cadherin and focal adhesion kinase in colorectal carcinoma and their clinical significances
- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 6
- DOI:
- 10.3969/j.issn.1009-3079.2007.06.009
- Key Word:
- 大肠癌;埃兹蛋白;黏着斑激酶;上皮细胞钙黏蛋白;侵袭;转移;免疫组化
Abstract： 目的:探讨大肠癌组织中埃兹蛋白(Ezrin),黏着斑激酶(FAK)及上皮细胞钙黏蛋白(E-cadherin)的表达及其与肿瘤侵袭和转移的关系.方法:大肠癌组织标本50例,其中高分化腺癌13例,中低分化腺癌37例;无淋巴结转移30例,淋巴结转移20例.采用免疫组织化学方法检测50例大肠癌组织中Ezrin,FAK及E-cadherin的蛋白表达,并运用统计学方法分析Ezrin,FAK及E-cadherin的表达与大肠癌各种临床病理特征的关系及三者的相关性.结果:Ezrin在中低分化、伴有淋巴结转移及Dukes C+D期大肠癌的阳性表达率显著高于高分化、无淋巴结转移及Dukes A+B期大肠癌(83.78% vs 46.15%,P＜0.01;95.00% vs60.00%,P＜0.01;95.00% vs 60.00%,P＜0.01),而E-cadherin在以上组织中的表达正好相反(24.32% vs 69.23%,P＜0.01;1 0.00% vs53.33%,P＜0.01;10.00% vs 53.33%,P＜0.01),其均与患者性别及年龄大小均无关(P＞0.05).FAK在Dukes C+D期、伴有淋巴结转移大肠癌组织中的阳性表达显著高于Dukes A+B、无淋巴转移组织(100.00% vs 63.33%,P＜0.01;100.00% vs 63.33%,P＜0.01),而与肿瘤的分化程度、患者性别及年龄大小均无关(P＞0.05).经Spearman相关分析,Ezrin与FAK在大肠癌组织中的表达呈正相关(r=0.346,P＜0.05),E-cadherin与Ezrin、FAK在大肠癌组织中的表达均呈明显负相关(r=-0.410,P＜0.01;r=-0.406,P＜0.01).结论:Ezrin,FAK及E-cadherin在大肠癌组织中的异常表达与肿瘤组织的浸润和转移密切相关,联合检测可以作为一组有效的大肠癌肿瘤标记和预后指标.
- [1]卢航青,郑杰.埃兹蛋白：生物学特征及其在肿瘤转移中的作用[J].2005,(3).
- [2]张志发,严群,黄志勇.黏附分子cadherin与肝癌生物学特性的关系[J].2006,(7).
- [3]杨红军,丁彦青.FAK在大肠癌中的表达及其临床意义[J].2003,(5).
- [4]姬宏飞,孟祥宁,傅松滨.FAK与肿瘤关系的研究进展[J].2005,(6).
- [5]刘启胜,于红刚,齐元玲,等.磷酸化黏着斑激酶在结肠癌中表达及意义[J].2005,(20).
- [6]J, Mathew,J E, Hines,J O, Obafunwa,etc.CD44 is expressed in hepatocellular carcinomas showing vascular invasion.[J].1996,179(1).
- [7]Birukov KG,Leitinger N,Bochkov VN,etc.Signal transduction pathways activated in human pulmonary endothelial cells by OxPAPC, a bioactive component of oxidized lipoproteins.[J].2004,67(1).
- [8]T.M Weiner,R.J Craven,W.G Cance,etc.Expression of focal adhesion kinase gene and invasive cancer[J].1993,342(8878).
- [9]Kaori Ohtani,Hideki Sakamoto,Thomas Rutherford,etc.Ezrin, a membrane-cytoskeletal linking protein, is involved in the process of invasion of endometrial cancer cells[J].1999,147(1-2).
- [10]Hunter KW.Ezrin, a key component in tumor metastasis.[J].2004,10(5).
- [11]Gautreau A.,Pujuguet P.,Del Maestro L.,etc.Ezrin regulates E-cadherin-dependent adherens junction assembly through Rac1 activation[J].2003,14(5).
- [12]Jacobson K,Roy P.Overexpression of profilin reduces the migration of invasive breast cancer cells.[J].2004,57(2).
- [13]Curto M,McClatchey AI.Ezrin...a metastatic detERMinant?[J].2004,5(2).
- [14]Meltzer PS,Helman L,Khanna C,etc.Expression profiling identifies the cytoskeletal organizer ezrin and the developmental homeoprotein Six-1 as key metastatic regulators.[J].2004,10(2).
- [15]Olomu O,Gorlick R,Helman LJ,etc.The membrane-cytoskeleton linker ezrin is necessary for osteosarcoma metastasis.[J].2004,10(2).
- [16]Jay DG,Roy C,Mcgarry L,etc.Essential Functions of Ezrin in Maintenance of Cell Shape and Lamellipodial Extension in Normal and Transformed Fibroblasts[J].1997,7(9).
- [17]Gonzalez MA,Pinder SE,Ellis IO,etc.An immunohistochemical examination of the expression of E-cadherin, alpha- and beta/gamma-catenins, and alpha2- and beta1-integrins in invasive breast cancer.[J].1999,187(5).
- [18]Parsons JT..Focal adhesion kinase: the first ten years[J].2003,116(8).