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Differentiation into hepatocytes and transplantation of bone marrow stromal stem cells

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Author:
No author available
Journal Title:
WORLD CHINESE JOURNAL OF DIGESTOLOGY
Issue:
5
DOI:
10.3969/j.issn.1009-3079.2007.05.006
Key Word:
骨髓基质干细胞;肝细胞;分化;移植

Abstract: 目的:研究骨髓基质干细胞(BMSCs)在体外向肝细胞的定向分化和体内移植后对小鼠受损肝脏的修复作用.方法:在体外培养体系中用肝细胞提取液(HE)模拟体内肝脏微环境,诱导BMSCs向肝细胞定向分化,以免疫细胞化学和吲哚靛青绿(ICG)染色检测其分化程度.将5-溴脱氧尿嘧啶核苷(BrdU)标记的BMSCs移植入小鼠体内,免疫组织化学方法显示BMSCs在肝脏的分布,血清酶学检测肝脏受损小鼠移植后7,14,28 d谷草转氨酶(AST,nkat/L)、谷丙转氨酶(ALT,nkat/L)和碱性磷酸酶(AKP,nkat/L)的含量变化.结果:在体外BMSCs可由HE诱导分化成肝细胞,但HE诱导组α1-抗胰蛋白酶(4 d:52.5±3.9 vs 76.8±4.5,P<0.05;14 d:60.3±6.1 vs85.4±7.6,P<0.05;21 d:80.5±8.0 vs 105.7±8.6,P<0.05)和白蛋白(4 d:43.2±6.5 vs 71.6±7.6,P<0.05;14 d:61.5±10.4 vs 93.6±13.9,P<0.05;21 d:80.6±17.1 vs 128.3±22.2,P<0.05)表达量少于HGF诱导组;体内移植后BMSCs整合于肝实质内;肝脏受损小鼠移植BMSCs后,血清中ALT(7 d:493.43±120.02 vs 696.81±140.03,P<0.01;14 d:558.45±130.03vs 780.16±151.7,P<0.01;28 d:583.45±138.36 vs 880.18±170.53,P<0.01)、AST(7 d:1521.97±186.7 vs 2342.14±208.38,P<0.01;14 d:1590.32±200.04 vs 2692.21±238.38,P<0.01;28 d:1625.33±208.38 vs 2872.24±281.72,P<0.01)和AKP(7 d:1.24±0.22 vs 1.78±0.18,P<0.01;14 d:1.21±0.21 vs 2±0.19,P<0.01;28 d:1.32±0.19 vs 2.27±0.2,P<0.01)比损伤组小鼠相应值均有下降,但仍高于正常对照组.结论:BMSCs在体外可由HE诱导分化成肝细胞,在体内移植后可促进小鼠受损肝脏的修复.

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