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Anti-angiogenic effect of nitric oxide synthase inhibitor on colorectal cancer xenografts in nude mice
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 2
- DOI:
- 10.3969/j.issn.1009-3079.2007.02.003
- Key Word:
- 大肠癌;N-硝基精氨酸甲酯;肿瘤血管形成
Abstract： 目的:观察一氧化氮合酶抑制剂N-硝基精氨酸甲酯(L-NAME)对大肠癌细胞的抑制作用,以及对移植瘤内血管生成的影响.方法:建立大肠癌裸鼠移植瘤模型再随机分为生理盐水对照组(n=6,0.2 mL)、L-NAME组(n=6,1.5 mg/kg)、5-FU组(n=6,5 mg/kg)、L-NAME+5-FU联合组(n=6,L-NAME 1.5 mg/kg,5-FU 5 mg/kg),皆以灌胃方式给药,3次/wk,共2 wk.测量各组肿瘤体积,观察L-NAME的体内抗瘤作用.采用免疫组化方法观察移植瘤的微血管密度(MVD),用Western blot方法检测肿瘤组织VEGF蛋白的表达.结果:L-NAME,5-FU,L-NAME+5-FU组抑瘤率分别为34.1%、46.0%、74.1%,与对照组比较,有显著性差异(P＜0.01),联合治疗组与L-NAME,5-FU组比较,差异也有显著性(P＜0.01);MVD值对照组为28.9±2.7,L-NAME组为16.2±3.1,与对照组比较,差异有显著性(P＜0.05);L-NAME组移植瘤VEGF蛋白的表达(113.14±2.34),与对照组(98.56±1.76)相比,明显减弱(P＜0.05).结论:L-NAME具有抑制肿瘤新生血管生成的作用,可抑制大肠癌裸鼠移植瘤的生长;和5-FU联合具有协同抗肿瘤作用.
- [1]徐梅华,邓长生,朱尤庆,等.诱导型一氧化氮合酶在结直肠肿瘤不同阶段中的表达[J].2003,(6).
- [2]Xin-Jie Niu,Zuo-Ren Wang,Sheng-li WU,etc.Relationship between inducible nitric oxide synthase expression and angiogenesis in primary gallbladder carcinoma tissue[J].2004,(5).
- [3]Long-Gang Li,Hui-Mian Xu.Inducible nitric oxide synthase, nitrotyrosine and apoptosis in gastric adenocarcinomas and their correlation with a poor survival[J].2005,(17).
- [4]Li-Bo Yu,Xin-Shu Dong,Wen-Zhou Sun,etc.Effect of a nitric oxide synthase inhibitor NG-nitro-L-arginine methyl ester on invasion of human colorectal cancer cell line SL-174T[J].2005,(40).
- [5]Orucevic A,Lala PK.NG-nitro-L-arginine methyl ester, an inhibitor of nitric oxide synthesis, ameliorates interleukin 2-induced capillary leakage and reduces tumour growth in adenocarcinoma-bearing mice[J].1996.
- [6]P K, Lala,C, Chakraborty.Role of nitric oxide in carcinogenesis and tumour progression.[J].2001,2(3).
- [7]Tanaka M,Uchiyama A,Mibu R,etc.Nitric oxide synthase expression and nitric oxide production in human colon carcinoma tissue.[J].1999,70(4).
- [8]Amila Orucevic,Peeyush K. Lala.Effects ofNG-Nitro-L-arginine Methyl Ester, an Inhibitor of Nitric Oxide Synthesis, on IL-2-Induced LAK Cell Generationin Vivoandin Vitroin Healthy and Tumor-Bearing Mice[J].1996,169(1).