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Down-regulated expression of PTEN in colorectal cancer and its clinical significance

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Author:: No author available

Journal Title:: WORLD CHINESE JOURNAL OF DIGESTOLOGY

Issue:: 28

DOI:: 10.3969/j.issn.1009-3079.2006.28.006

Key Word:: 结直肠癌;癌旁组织;PTEN基因;转移

Abstract：目的:研究PTEN在结直肠癌中的表达及其与结直肠癌病理特征的关系.方法:应用免疫组化SP法分别检测65例癌组织和癌旁组织及13例腺瘤组织PTEN蛋白的表达.结果:PTEN主要在细胞核或细胞质中表达,在结直肠癌组织中阳性表达率显著低于癌旁组织阳性表达率(56.92% vs 86.15%,P＜0.01).中、高分化腺癌阳性表达率显著高于低、未分化腺癌阳性表达率(75.76% vs 37.50%,P＜0.01).Dukes A、B期阳性表达率显著高于Dukes C、D期阳性表达率(73.33% vs 42.86%,P＜0.05).此外,PTEN的表达与淋巴转移有关(P＜0.01),而与性别、年龄、同期的肿瘤大小无关.结论:PTEN在结直肠癌组织中的表达下调,其表达水平可作为反映结直肠进展和预后的生物学指标之一.

Reference

[1]张亚历,张振书,吴保平,等.大肠癌相关标记物筛检高危人群的评价[J].2000,(10).
[2]白伟良,李巍,陈晓秋,等.蛋白酪氨酸磷酸酶基因启动子过甲基化与喉癌的关系[J].2006,(6).
[3]肖秀英,周晓燕,孙孟红,等.散发性结直肠癌中微卫星不稳定性及临床病理意义[J].2006,(4).
[4]张立力.多原发大肠癌微卫星不稳定性的研究[J].1999,(5).
[5]Tomohiko Maehama,Jack E. Dixon.PTEN: a tumour suppressor that functions as a phospholipid phosphatase[J].1999,9(4).
[6]Li, Jing,Yen, Clifford.PTEN, a putative protein tyrosine phosphatase gene mutated in human brain, breast, and prostate canc[J].1997,275(5308).
[7]Dean M,Ali IU,Schriml LM.Mutational spectra of PTEN/MMAC1 gene: a tumor suppressor with lipid phosphatase activity.[J].1999,91(22).
[8]Hanna, Romanowicz-Makowska,Beata, Smolarz,Ewa, Langner,etc.Analysis of microsatellite instability and BRCA1 mutations in patients from hereditary nonpolyposis colorectal cancer (HNPCC) family.[J].2005,56(1).
[9]Codogno P,Arico S,Meijer AJ,etc.The tumor suppressor PTEN positively regulates macroautophagy by inhibiting the phosphatidylinositol 3-kinase/protein kinase B pathway.[J].2001,276(38).
[10]I W, Cheney,D E, Johnson,M T, Vaillancourt,etc.Suppression of tumorigenicity of glioblastoma cells by adenovirus-mediated MMAC1/PTEN gene transfer.[J].1998,58.
[11]Jie-Oh Lee,Haijuan Yang,Maria-Magdalena Georgescu,etc.Crystal Structure of the PTEN Tumor Suppressor: Implications for Its Phosphoinositide Phosphatase Activity and Membrane Association[J].1999,99(3).
[12]Joyce KA,Halliwell N,Roebuck MM,etc.Mutation in the PTEN/MMAC1 gene in archival low grade and high grade gliomas.[J].1999,79(9).
[13]Hanafusa H,Shishido T,Kirsch KH,etc.The tumor-suppressor activity of PTEN is regulated by its carboxyl-terminal region.[J].1999,96(18).
[14]Kang YoungHwa,Lee HyeSeung,Kim WooHo.Promoter methylation and silencing of PTEN in gastric carcinoma.[J].2002,82(3).
[15]Maehama T,Dixon JE.The tumor suppressor, PTEN/MMAC1, dephosphorylates the lipid second messenger, phosphatidylinositol 3,4,5-trisphosphate.[J].1998,273(22).
[16]Yamada KM,Takino T,Miyamoto S,etc.PTEN interactions with focal adhesion kinase and suppression of the extracellular matrix-dependent phosphatidylinositol 3-kinase/Akt cell survival pathway.[J].1999,274(29).
[17]D M, Li,H, Sun.TEP1, encoded by a candidate tumor suppressor locus, is a novel protein tyrosine phosphatase regulated by transforming growth factor beta.[J].1997,57.
[18]Yamada KM,Gu J,Tamura M.Tumor suppressor PTEN inhibits integrin- and growth factor-mediated mitogen-activated protein (MAP) kinase signaling pathways.[J].1998,143(5).
[19]Haier J,Nicolson GL.Time-dependent dephosphorylation through serine/threonine phosphatases is required for stable adhesion of highly and poorly metastatic HT-29 colon carcinoma cell lines to collagen.[J].2000,20(4).
[20]Gao XS.,Pan DJ.,Neufeld TP..Drosophila PTEN regulates cell growth and proliferation through PI3K-dependent and -independent pathways[J].2000,221(2).
[21]Ross JS,Rosenthal SI,Depowski PL.Loss of expression of the PTEN gene protein product is associated with poor outcome in breast cancer.[J].2001,14(7).
[22]Nystrom Lahti M,Peltomaki P,Zhou XP,etc.Distinct PTEN mutational spectra in hereditary non-polyposis colon cancer syndrome-related endometrial carcinomas compared to sporadic microsatellite unstable tumors.[J].2002,11(4).