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Deregulation of cyclin E induces chromosomal instability in human colon cancer

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Author:
No author available
Journal Title:
WORLD CHINESE JOURNAL OF DIGESTOLOGY
Issue:
22
DOI:
10.3969/j.issn.1009-3079.2006.22.003
Key Word:
Cyclin E;人类结肠癌;CIN

Abstract´╝Ü 目的:研究Cyclin E的表达失调对人类结肠癌细胞系DLD1染色体稳定性的影响.方法:建立四环素调控的Cyclin E稳定表达的细胞系DLD1tTA-Cvclin E,Western blot检测CyclinE表达.CyclinE持续稳定表达1,3,5,7和14 d后,对细胞进行DAPI染色,在荧光显微镜下计数CIN细胞的百分比.结果:Western blot显示,Cyclin E表达在96 h后达最高峰.Cyclin E表达关闭的DLDl细胞中,CIN细胞的百分比波动在0.97%-1.22%之间(t=3.81,P>0.01);而CycIin E持续表达1,3,5,7和14 d后的DLD1细胞中,CIN细胞的百分比分别为2.41%,3.63%,3.92%,6.17%和8.34%(t=4.77,P<0.01).结论:Cyclin E的表达失调可导致DLDl结肠癌细胞非整倍体数染色体的形成,也是造成结肠癌CIN的重要因素.

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