Effects of spent culture supernatant of Lactobacillus acidophilus on intestinal flora in mice with antibiotic-associated diarrhoea
- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 19
- DOI:
- 10.3969/j.issn.1009-3079.2006.19.004
- Key Word:
- 嗜酸性乳杆菌;耗尽培养上清液;抗生素相关性腹泻;肠道菌群
Abstract： 目的:研究嗜酸性乳杆菌LAl4菌株耗尽培养上清液(spent culture supernatant,SCS)对抗生素相关性腹泻小鼠肠道菌群的影响.方法:小鼠60只,随机分为正常组、模型组、SCS组、活菌组、SCS+活菌组及自然恢复组(每组n=10).采用ip氨苄青霉素2000 mg/(kg·d),连续3d造成肠道菌群失调性腹泻动物模型.正常组和模型组小鼠于造模后即处死,取盲肠内容物进行肠道菌群分析.其余各组小鼠分别灌胃受试药物或生理盐水30mL/(kg·d)[含菌组中活菌数量为3×109CFU/(kg·d)],3d后处死,取盲肠内容物进行肠道菌群分析.结果:小鼠ip氨苄青霉素造成肠道菌群失调,肠道内4种主导菌群的数量(1gCFU/L)明显改变,与正常组比较肠杆菌和肠球菌数量显著上升(10.13±0.10 vs 9.03±0.11,10.52±0.11 vs 9.11±0.09,P＜0.01),乳杆菌和双歧杆菌数量明显下降(10.51±0.07 vs 11.88±0.10,P＜0.01;10.38±0.31 vs 11.61±0.13,P＜0.05).而灌胃给予SCS、活菌、SCS加活菌后,与自然恢复组比较肠道乳杆菌和双歧杆菌数量明显回升(乳杆菌:11.53±0.17 vs 9.74±0.37,P＜0.01;11.54±0.05,11.45±0.07 vs 9.74±0.37,P＜0.05;双歧杆菌:11.54±0.22,11.30±0.99 vs 9.51±0.52,P＜0.05;11.13±0.16 vs 9.51±0.52,P＜0.01),肠杆菌和肠球菌数量下降(肠杆菌:9.42±0.22,9.50±0.06,9.22±0.39 vs 9.97±0.61,P＜0.05;肠球菌:9.48±0.20,9.45±0.16,9.37±0.21 vs 9.89±0.43,P＜0.05).结论:嗜酸性乳杆菌活菌和SCS均能调整抗生素相关性腹泻小鼠肠道菌群失调,对乳杆菌和双歧杆菌有显著扶持作用.
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