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Inhibitory effect of Survivin antisense oligonucleotide on growth of human hepatic carcinoma transplanted subcutaneously

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Author:
No author available
Journal Title:
WORLD CHINESE JOURNAL OF DIGESTOLOGY
Issue:
16
DOI:
10.3969/j.issn.1009-3079.2006.16.016
Key Word:
反义寡核苷酸;肝癌;Survivin;疾病模型;裸鼠

Abstract: 目的:检测Survivin反义寡核苷酸(ASODN)在人肝癌耐药细胞株裸鼠皮下移植瘤中的表达情况.方法:将30只裸鼠建立人肝癌耐药细胞系SMMC-7721/ADM皮下移植瘤模型,随机分成6组:空白对照组(A)、脂质体转染对照组(B)、正义链对照组(C)、200(D)、400(E)和600μg/L(F)反义链组(ASODN组),用不同的转染液注射后2,4,8,12,16,20 d,用逆转录聚合酶链反应技术(RT-PCR)和Western blot蛋白免疫印迹法检测治疗后各组肿瘤组织中SurvivinmRNA和蛋白表达的变化.结果:注射后20 d,ASODN组肿瘤细胞生长明显抑制,空白对照组、脂质体转染对照组和正义链对照组裸鼠的mRNA和蛋白表达无明显差异,而ASODN组mRNA和蛋白表达随着时间和浓度的增加,Survivin表达减弱,E,F组与其余个组(A,B,C,D)相比有显著差异(mRNA:0.33±0.04,0.28±0.03vs 0.82±0.02,0.78±0.05,0.72±0.04,0.57±0.03,P<0.05;蛋白:34.9±3.89,21.2±3.65 vs 72.14±6.53,69.31±5.34,68.29±4.98,53.8±5.23,P<0.05).结论:Survivin反义寡核苷酸能够下调Survivin mRNA和蛋白的表达,抑制裸鼠皮下移植瘤的生长.

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