Changes of endotoximia,cytokines and nitric oxide and therapeutic effects of sandostatin in rats with severe acute pancreatitis
- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 15
- DOI:
- 10.3969/j.issn.1009-3079.2006.15.017
- Key Word:
- 胰腺炎;内毒素;肿瘤坏死因子;白细胞介素6;一氧化氮;过氧化脂质;善宁
Abstract： 目的:探讨内毒素血症、细胞因子和一氧化氮在重症急性胰腺炎(severe acute pancreatitis,SAP)发展中的作用及善宁的治疗作用.方法:经胰管逆行注射15 g/L去氧胆酸钠建立SAP模型,给予善宁治疗,观察造模后各时间点血中内毒素(ET)、过氧化脂质(LPO),TNF-α,IL-6,一氧化氮(NO)的变化,并进行相关分析;于24 h后取胰腺组织,测定胰腺组织中LPO,TNF-α,IL-6和NO水平,同时进行相关分析及胰腺组织病理学检查.结果:与对照组比较,SAP组各时间点血浆ET,LPO,TNF-α,IL-6,NO的水平明显升高(P＜0.01),其中ET,LPO,TNF-α和NO 6 h尤为显著(898±114 EU/L,24.58±1.23 μmol/L,246.3±16.5 ng/L,162.8±10.9 mmol/L,P＜0.05vs12,24 h).胰腺组织LPO,TNF-α,IL-6,NO的水平SAP组明显高于对照组(3.31±0.85μmol/g vs 0.33±0.04 μmol/g,P＜0.01;2.57±0.14 ng/g vs 0.16±0.04 ng/g,P＜0.01;85.6±24.6 ng/g vs 32.5±5.7 ng/g,P＜0.01;15.3±1.2 mmol/g vs 6.6±1.4 mmol/g,P＜0.01),光镜下胰腺组织病理损害明显.相关分析显示SAP组血浆LPO,TNF-α,IL-6,NO水平分别与ET水平呈显著正相关(r=0.858,P＜0.01;r=0.958,P＜0.01;r=0.918,P＜0.01;r=0.875,P＜0.01).善宁治疗后上述各指标均较SAP组明显改善(P＜0.01).结论:ET血症、细胞因子、NO等相互激发、相互促进,在SAP的发展中起着重要的协同作用.善宁能阻断这些损伤因子的连锁反应.
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