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Effects of intraperitoneal injection of botulinum toxin A on passage velocity in small intestine and expression of acetylcholinesterase and substance P in rats

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Author:
No author available
Journal Title:
WORLD CHINESE JOURNAL OF DIGESTOLOGY
Issue:
14
DOI:
10.3969/j.issn.1009-3079.2006.14.015
Key Word:
A型肉毒毒素;小肠通过速度;胆碱酯酶;P物质;大鼠

Abstract´╝Ü 目的:研究A型肉毒毒素(botulinum toxin A,BTA)腹腔注射(ip)对大鼠小肠通过速度及胆碱酯酶(AChE)和P物质(substance P,SP)表达的影响.方法:8周龄Wistar♂大鼠24只随机分为A,B,C,D4组,每组6只;A,B,C组分别ip含BTA 4,2,1 U的生理盐水2 mL,D组为对照组,ip生理盐水2 mL.4 wk后行小肠碳沫推进实验,取回肠末段标本行免疫组织化学染色对比AChE及SP的表达.结果:A,B组大鼠的小肠碳沫推进百分比(分别为53.9%±4.0%和53.2%±3.0%)小于D组(66.4%±5.6%;P<0.05),但C组(64.3%±5.6%)与D组无统计学差异;A,B组AChE和SP在回肠肌层及黏膜层表达减少,整合光密度与D组相比差异显著(肌层AChE:99.3±39.3,76.3±36.4 vs 260.1±114.8,P<0.05;黏膜层AChE:224.6±85.4,230.1±120.7 vs 230.1±120.7,P<0.05:肌层SP:161.2±90.6,200.7±65.5 vs355.9±70.9,P<0.05;黏膜层SP:198.6±60.3,229.7±71.8 vs 412.5±79.2,P<0.05),C组与D组无统计学差别(P>0.05).结论:BTA ip能减慢大鼠的小肠碳沫推进速度,减少回肠末段AChE和SP的表达.

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