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In vivo expression of hepatocyte growth factor promotes differentiation of stem cells from umbilical cord blood into hepatocyte-like cells
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 8
- DOI:
- 10.3969/j.issn.1009-3079.2006.08.006
- Key Word:
- 肝细胞生长因子;造血干细胞;肝细胞
Abstract： 目的:观察肝细胞生长因子(hepatocyte growth factor,HGF)作用下,脐血干细胞在体内向肝细胞分化的情况.方法:HGF的裸DNA质粒通过尾静脉快速注入6-8周龄的非肥胖糖尿病/重症联合免疫缺陷(NOD/SCID)小鼠体内,酶联免疫吸附法(ELISA)检测外周血中HGF水平.收集足月妊娠产妇的脐血,磁式分选法选出CD34+造血干细胞(hematopoietic stem cell,HSC).20 μL四氯化碳(carbon tetrachloride,CCl4)腹腔注射建立NOD/SCID小鼠急性肝损伤模型,分别输注CD34+HSC和/或HGF质粒,观察其对死亡率、肝功能恢复的影响,并检测小鼠肝组织内的人源性细胞.结果:快速注入HGF质粒后,体内HGF水平明显上升.实验各组存活率及肝功能恢复情况无明显差异.病理切片显示,联合应用HGF+HSC的实验组,其肝组织损伤程度最轻,单独应用HGF或HSC的两个实验组结果类似,对照组最重.在两组注射HSC的小鼠肝组织中,均可检测到人源性的、分泌白蛋白的肝样细胞,且联合应用HGF的实验组中,此种细胞更多,分布更广.结论:HGF可以促进脐血干细胞向肝细胞分化,并发挥肝细胞功能.
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