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Relations of connexin 32 and 43 expression with metastatic potential of gastric cancer
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 6
- DOI:
- 10.3969/j.issn.1009-3079.2006.06.003
- Key Word:
- 连接蛋白;间隙连接通讯;胃癌;转移潜能
Abstract： 目的:研究间隙连接蛋白Cx32,Cx43表达水平对胃癌侵袭转移潜能的影响.方法:采用免疫组化SP法和间接免疫荧光方法分别检测Cx32,Cx43在正常胃组织及不同病理分型的胃癌组织和不同分化程度胃癌细胞株中的表达,并分析该蛋白表达对胃癌侵袭转移潜能的影响.结果:免疫组化SP法结果显示:Cx32,Cx43阳性棕黄色颗粒位于细胞膜和细胞质内,在正常胃组织中的阳性表达率显著高于胃癌组织(Cx32:χ2=23.1,P＜0.05,Cx43:χ2=11.6,P＜0.05),Cx32,Cx43在高中分化胃癌的阳性表达率显著高于低分化胃癌(Cx32:χ2=32.1,P＜0.05;Cx43:χ2=22.6,P＜0.05).间接免疫荧光法结果显示:在转化的人胃细胞系(GES-1)、胃高分化腺癌细胞系(N87)中Cx32,Cx43的表达均为阳性,但表达率和表达部位有显著差异.在GES-1中Cx32,Cx43绿色荧光沿其细胞膜呈颗粒状或细线状分布,其阳性表达率均为100%;在N87中,Cx32绿色荧光沿其细胞膜呈颗粒状或细线状分布,其阳性表达率为49%,而Cx43荧光分布在其细胞质中,其阳性表达率为55%;在BGC-823中Cx32,Cx43的表达均为阴性.结论:Cx32,Cx43是影响胃癌转移潜能的一个重要因素.
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