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Isolation of differentially methylated DNA sequences of gastric cancer by methylation-sensitive representational difference analysis
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 4
- DOI:
- 10.3969/j.issn.1009-3079.2006.04.002
- Key Word:
- 胃癌;DNA甲基化;差异片段;MS-RDA
Abstract： 目的:应用甲基化敏感性代表性差异分析(methylation-sensitive representational difference analysis,MS-RDA)筛选胃癌和正常胃组织间甲基化差异DNA片段.方法:通过MS-RDA筛选胃癌和正常胃组织间DNA甲基化差异片段,经克隆、测序后进行生物信息学分析.结果:获得3个甲基化差异片段,分别为CRS1308,CRS1309,CRS1310序列,其中CRS1309和CRS1310已被GenBank收录,登陆号分别为AY887106和AY887107,CRS1309序列与LOC440683基因第11外显子、LOC440887基因的3'端,DRD5基因启动子和外显子区域均有很高的相似性(分别为98%,99%,94%),CRS1310序列与1999年Minory Toyota在人类结肠癌中分离出的核糖体RNA上的甲基化差异性CpG岛有很高的相似性(98%).结论:胃癌和正常胃组织间DNA甲基化存在差异,MS-RDA可有效分析这两种不同组织间甲基化的差异,筛选出有意义的甲基化差异片段.
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