Effects of traditional Chinese medicine Ganyanping on TGFβ1/ Smad signaling pathway in carbon tetrachloride-induced hepatic fibrosis of rats
- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 2
- DOI:
- 10.3969/j.issn.1009-3079.2006.02.005
- Key Word:
- 肝炎平;TGFβ1/Smad信号通路;肝纤维化
Abstract： 目的:研究中药肝炎平对CCl4诱导的大鼠肝纤维化肝脏TGFβ1及其受体TβR Ⅰ,TβRⅡ水平和Smad3,Smad7及前胶原α2(Ⅰ)(Colla2)mRNA表达的影响,探讨其抗纤维化的作用机制.方法:健康Wistar大鼠38只随机分为正常对照组(N组)、模型对照组(A组)以及肝炎平治疗组(B组).N组每日以生理盐水10μL/g灌胃,A组及B组用CCl4制备大鼠肝纤维化模型,B组4 wk后开始以肝炎平10 μL/g灌胃给药,A组同时以生理盐水10μL/g灌胃,每日1次.9 wk后处死大鼠,取肝组织作病理学检查,免疫组化检测TGFβ1及其受体TβR Ⅰ,TβRⅡ的表达,RT-PCR检测胞内信号蛋白Smad3,Smad7以及Colla2mRNA的表达.结果:与正常对照组相比,模型组TGFβ1、TβR Ⅰ、TβRⅡ、Smad3、Colla2表达明显增强,Smad7表达明显下降.经肝炎平治疗后大鼠肝脏TGFβ1及其受体TβR Ⅰ,TβRⅡ表达均比模型组减弱(TGFβ1:4.30±0.16 vs 4.18±0.17,P＜0.05;TβR Ⅰ:4.35±0.14 vs 4.24±0.10,P＜0.05;TβRⅡ:4.37±0.11 vs 4.27±0.08,P＜0.05).肝炎平同时下调Smad3和Colla2mRNA的表达(Smad3:0.93±0.05 vs 0.90±0.41,P＜0.05;Colla2:0.95±0.03 vs 0.92±0.03,P＜0.05),上调Smad7 mRNA的表达(0.84±0.05vs 0.87±0.03,P＜0.05).另外,肝炎平组大鼠肝脏病理变化显著改善.结论:肝炎平对大鼠肝纤维化肝脏TGFβ1/Smad信号通路有明显的影响,能抑制CCl4诱导的大鼠肝纤维化,其作用机制可能为抑制TGFβ1及其受体TβR Ⅰ,TβRⅡ蛋白表达,下调Smad3 mRNA,上调Smad7 mRNA的表达,并最终下调了作为主要细胞外基质的Colla2mRNA的表达.
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