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Effect of octreotide on contraction of rat hepatic stellate cell
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 1
- DOI:
- 10.3969/j.issn.1009-3079.2006.01.015
- Key Word:
- 奥曲肽;内皮素-1;肝星状细胞;胞质游离钙
Abstract： 目的:探讨奥曲肽(OCT)对内皮素-1(ET-1)刺激的肝星状细胞(HSC)收缩的影响,以及此过程中胞质游离钙([Ca2+]i)的变化.方法:应用体外HSC培养技术,借助激光共聚焦显微镜(LSCM),采用第三代钙荧光探针Fluo-3/A1M观察OCT、ET-1介导的HSC[Ca2+]i荧光强度、细胞面积及最大长径的变化.结果:2.5 mg/L OCT对HSC内Ca2+浓度及收缩无显著影响;5.0 mg/L、10.0 mg/L组OCT能够即刻引起HSC内Ca2+浓度显著下降,10 min时下降率分别为36.59%、39.13%(P＜0.05),但对HSC收缩无显著影响.ET-1引起HSC内Ca2+浓度短暂而明显的升高以及HSC显著的收缩.5.0 mg/L、10.0 mg/L组OCT能够抑制ET-1引起的HSC内Ca2+浓度升高及收缩作用(P＜0.05).结论:OCT可即刻引起HSC内Ca2+浓度下降,但对HSC收缩影响不大.OCT能够抑制ET-1引起的HSC收缩.
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