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Effects of tea polyphenols on alcoholic liver injury in rats

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Author:: No author available

Journal Title:: WORLD CHINESE JOURNAL OF DIGESTOLOGY

Issue:: 1

DOI:: 10.3969/j.issn.1009-3079.2006.01.010

Key Word:: 酒精性肝病;茶多酚;EGCG;内毒素血症;细胞因子

Abstract：目的:研究绿茶多酚及表没食子儿茶素没食子酸酯(EGCG)对大鼠酒精性肝损伤的治疗作用,并探讨其作用机制.方法:将48只大鼠随机分为正常对照组、模型组、茶多酚治疗Ⅰ组、Ⅱ组、EGCG治疗Ⅱ组、Ⅱ组,每组8只大鼠.以酒精+0.5 mL鱼油灌胃制作酒精性肝病模型.茶多酚治疗Ⅰ、Ⅱ组另外分别给予200 mg/kg、100 mg/kg茶多酚灌胃治疗,EGCG Ⅰ、Ⅱ治疗组则分别给予100 mg/kg、50 mg/kg EGCG灌胃治疗.5 wk后处死大鼠,观察各组大鼠肝脏病理变化,并测定血清转氨酶及血浆内毒素水平,采用ELISA法检测血清TNF-α、IL-1、IL-18水平,并应用RT-PCR方法检测大鼠肝组织CD14、LBP、TNF-α、IL-1、IL-18 mRNA的表达,并应用图像分析系统对其进行半定量分析.结果:模型组大鼠肝组织表现肝细胞中度脂肪变性,点状坏死,炎性细胞浸润,其血清ALT、TNF-α、IL-1、IL-18及血浆内毒素水平与正常对照组相比,显著升高(P＜0.05或P＜0.01);模型组肝组织CD14、LBP、TNF-α、IL-1、IL-18 mRNA的表达与对照组相比显著增强(P＜0.05或P＜0.01).茶多酚、EGCG高低剂量分别同时处理显著降低了酒精性肝损伤大鼠血清ALT、TNF-α、IL-1、IL-1 8与血浆内毒素水平及肝组织CD14、LBP、TNF-α、IL-1、IL-18 mRNA的表达(P＜0.05或P＜0.01),肝组织仍可见肝细胞脂肪变性,但未见坏死及炎性细胞浸润.结论:茶多酚及EGCG可减轻酒精性肝损伤大鼠肝脏的炎症与坏死,其可能机制包括降低内毒素血症,抑制Kupffer细胞活性与促炎细胞因子的表达与分泌.

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