You Position: Home > Paper
Effects of matrine on apoptosis of hepatocytes and expression of regulating gene during cold preservation and reperfusion injury in rat donor liver
( views:201, downloads:4 )
- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 1
- DOI:
- 10.3969/j.issn.1009-3079.2006.01.005
- Key Word:
- 肝移植;苦参碱;再灌注损伤;凋亡;大鼠
Abstract： 目的:探讨苦参碱对大鼠原位肝移植供肝冷保存再灌注损伤中肝细胞凋亡及调控基因表达的影响.方法:应用延长保存的大鼠原位肝移植模型,大鼠84只随机分为对照组、低剂量苦参碱治疗组(40 mg/kg)、高剂量苦参碱治疗组(80mg/kg)和假手术组,将供肝在4℃林格液中保存5 h后植入受体,各组大鼠于再灌注后4和24 h后取样.采用TUNEL法分别检测移植术后肝细胞凋亡,流式细胞仪检测凋亡相关基因Bcl-2,FasL蛋白的表达,光镜下观察移植肝脏病理形态学的改变.结果:与对照组比较,苦参碱低、高剂量治疗组术后肝脏细胞凋亡指数显著降低(6.07±1.68,6.17±0.83 vs 14.87±2.10,P＜0.01),肝组织Bcl-2表达增加(59.32±14.09,58.90±16.70vs 17.00±8.01,P＜0.01),但FasL表达量无显著差异(P＜0.05),肝细胞凋亡指数、Bcl-2和FasL表达在苦参碱低、高剂量组间也无显著差异.对照组肝细胞损伤的病理表现相当严重,而治疗组的病理表现显著改善.结论:苦参碱通过促进抑制凋亡基因Bcl-2的表达来抑制冷保存再灌注导致的肝细胞凋亡.
- [1]Xin-Hua Zhu,Yu-Dong Qiu,Hao Shen,etc.Effect of matrine on Kupffer cell activation in cold ischemia reperfusion injury of rat liver[J].2002,(6).
- [2]王轩,杨甲梅,严以群,等.大鼠原位肝移植模型术式的改进[J].1997,(1).
- [3]F, Carlucci,E, Marinello,G, Gerunda,etc.Liver transplant: adenosine metabolism and apoptosis.[J].2004,23(8-9).
- [4]张俊平,张珉,周俭平,等.苦参碱体内外抗大鼠肝纤维化的作用[J].2001,(2).
- [5]叶明.苦参碱注射液的药理研究[J].1997,(11).
- [6]Teoh NC,Farrell GC.Hepatic ischemia reperfusion injury: Pathogenic mechanisms and basis for hepatoprotection.[J].2003,18(8).
- [7]Zhu X,Ding Y,Shi M,etc.Matrine protects sinusoidal endothelial cells from cold ischemia and reperfusion injury in rat orthotopic liver transplantation.[J].2003,33(2).
- [8]Kienle K,Rentsch M,Beham A,etc.Caspase 3 inhibition improves survival and reduces early graft injury after ischemia and reperfusion in rat liver transplantation.[J].2004,78(9).
- [9]G, Bilbao,J L, Contreras,D E, Eckhoff,etc.Reduction of ischemia-reperfusion injury of the liver by in vivo adenovirus-mediated gene transfer of the antiapoptotic Bcl-2 gene.[J].1999,230.
- [10]E S, Baskin-Bey,A, Canbay,S F, Bronk,etc.Cathepsin B inactivation attenuates hepatocyte apoptosis and liver damage in steatotic livers after cold ischemia-warm reperfusion injury.[J].2005,288(2).