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Culture of nanobacteria from human gallbladder bile
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 22
- DOI:
- 10.3969/j.issn.1009-3079.2005.22.007
- Key Word:
- 胆囊结石;纳米细菌;细胞培养;电子显微镜;间接免疫荧光染色
Abstract： 目的:介绍从胆汁中培养纳米细菌的方法.方法:无菌抽取30例胆囊结石患者的胆汁经"稀释-离心-过滤"或"过滤法"预处理后,在常规细胞培养条件下进行纳米细菌培养.光镜下观察细菌形态.采用间接免疫荧光染色对培养所得细菌进行鉴定,并在电镜下观察.结果:30例胆汁样本,经过"稀释-离心-过滤"预处理后,纳米细菌培养的阳性率为40%,经"过滤法"预处理后,纳米细菌培养的阳性率为57%,两种方法预处理后,胆汁纳米细菌培养阳性率无统计学差异(χ2=1.669,P＞0.05).培养2 wk时,显微镜下可见做布朗运动的微小的颗粒状纳米细菌.4 wk时,可见纳米细菌开始贴附于培养瓶底部形成细菌被膜.培养所得纳米细菌与8D10抗体产生特异性结合,电镜下观察呈球形或短棒状颗粒,大小约为80-350 nm.结论:在胆囊结石患者的胆汁中存在纳米细菌感染,"过滤法"是一种简单有效的预处理胆汁的方法.
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