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Roles of leptin and stearoyl-CoA desaturase-1 in pathogenesis of nonalcoholic fatty liver disease induced by a fat-rich diet
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 19
- DOI:
- 10.3969/j.issn.1009-3079.2005.19.010
- Key Word:
- 高脂饮食;非酒精性脂肪肝;罗格列酮;瘦素;硬脂酰CoA去饱和酶-1
Abstract： 目的:研究瘦素及SCD-1在高脂饮食引起非酒精性脂肪肝形成及其药物治疗中的作用.方法:42只SD大鼠分成正常组、高脂组和干预组(自高脂饮食喂养8 wk起用罗格列酮进行干预16 wk).酶联免疫法测定血清瘦素,RT-PCR实时荧光分析大鼠肝SCD-1 mRNA与β-actin mRNA的比值.结果:肝组织HE染色显示高脂组大鼠肝脏内有弥漫性肝细胞脂肪变性,8 wk达到脂肪肝诊断标准.8,24wk高脂组大鼠血清瘦素水平升高,与正常组相比有显著性差异(8 wk:5.29±1.83μg/L vs 3.06±1.35 μg/L,P＜0.05;24 wk:7.89±3.01 μg/L vs 3.09±1.52μg/L,P＜0.05);肝SCD-1 mRNA与β-actin mRNA的比值明显下降(8 wk:0.37±0.25 vs 0.82±0.34,P＜0.05).干预组与高脂纽相比,血清瘦素水平下降(5.95±3.31 μg/L vs 7.89±3.0l g/L,P＞0.05);肝SCD-1 mRNA表达明显增强(SCD-1/β-actin:1.02±0.11 vs 0.52±0.22,P＜0.01).结论:长期高脂饮食可导致血清瘦素水平升高,其通过下调肝SCD-1表达促进非酒精性脂肪肝的形成.罗格列酮可降低血清瘦素水平,上调肝SCD-1的表达,减轻因高脂饮食引起的非酒精性肝脂肪变.
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