Effects of glutamine on glutathione content and expression of Bcl-2 and Bax protein during hepatic ischemia and reperfusion injury in rats
- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 19
- DOI:
- 10.3969/j.issn.1009-3079.2005.19.003
- Key Word:
- 谷氨酰胺;肝脏缺血再灌注损伤;谷胱甘肽;Bcl-2;Rax
Abstract： 目的:探讨谷氨酰胺(glutamine,Gln)对大鼠肝脏缺血再灌注损伤(HIRI)时肝组织谷胱甘肽(glutathione,GSH)含量和细胞凋亡相关基因Bcl-2和Bax蛋白表达的影响.方法:将48只健康♂ Wistar大鼠随机分为谷氨酰胺组(G组)和对照组(C组).预置中心静脉导管后经此输液通道分别注入谷氨酰胺溶液和生理盐水行预处理(3 d).采用Pringle法夹闭肝十二指肠韧带致肝脏缺血30 min后,去夹恢复血流为再灌注.分别于再灌注后1、24 h抽血检测ALT的水平,然后快速切取肝组织检测其还原型GSH的含量,切取部分肝组织用于组织病理学检查,并采用S-P免疫组织化学染色方法检测肝组织细胞凋亡相关基因Bcl-2和Bax的蛋白表达情况.结果:再灌注后1、24 h,G组血清ALT水平皆显著低于C组(8.3±2.0 μkat/L vs 13.7±5.5 μkat/L,P＜0.05;2.9±2.5 μkat/L vs 9.1±4.3 μkat/L,P＜0.01).肝脏组织GSH的水平:再灌注后1、24 h,G组肝组织GSH水平皆明显高于C组(1216.09±152.78 μg/g vs 856.68±117.64μg/g,P＜0.01;899.73±57.75 μg/g vs 800.50±94.79μg/g,P＜0.05).肝组织损害的病理学改变:G组明显轻于C组.再灌注后1、24 h,G组肝组织Bcl-2蛋白阳性表达率明显高于C组(100.0% vs 37.5%,P＜0.05;87.5% vs 25.0%,P＜0.05);再灌注后1、24 h,G组肝组织Bax蛋白阳性表达率明显低于C组(25.0% vs 87.5%,P＜0.05;25.0% vs 87.5%,P＜0.05).结论:Gln对大鼠HIRI具有保护作用,其作用机制可能与维持体内GSH含量和影响肝组织细胞凋亡相关基因Bcl-2和Bax蛋白的表达有关.
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