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Pathological examination of mesenchymal stem cells homing to liver during allografting in rats
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 10
- DOI:
- 10.3969/j.issn.1009-3079.2005.10.008
- Key Word:
- 骨髓间质干细胞;绿色荧光蛋白;移植
Abstract： 目的:探索大鼠骨髓间质干细胞(MSC),在同种异体移植后受体大鼠肝脏的定居分布情况.方法:以绿色荧光蛋白标记从大鼠骨髓中分离培养的MSC,分别从大鼠尾静脉和门静脉注入同种异体正常和肝脏损伤大鼠体内,于移植后的第7 d,分别完整取肝,通过荧光检测和常规病理、免疫组织化学检查,研究MSC在大鼠肝脏内定居分布情况.结果:经不同途径移植MSC,在受体大鼠肝脏内均有定居.在肝脏受损的大鼠肝内,其已成功地分化为成熟的肝细胞和卵圆细胞,并能长期存活,在肝脏未受损的大鼠,MSC仅少量分化为卵圆细胞,所移植的MSC定居于肝脏和是否分化为成熟肝细胞、卵圆细胞与其移植途径无明显关系,与肝脏是否受损伤相关.结论:植入MSC是肝脏卵圆细胞的细胞来源,并且可以分化为成熟的肝细胞.干细胞定居于肝脏数量与肝脏是否受损伤关系密切,与移植途径无关.
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