Relationship between Epstein-Barr virus and overexpression of c-Met protein in gastric carcinoma
- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 3
- DOI:
- 10.3969/j.issn.1009-3079.2005.03.010
- Key Word:
- EB病毒;胃癌;扩增;c-Met基因;c-Met蛋白
Abstract： 目的:研究EBV相关胃癌(EBV associated gastric carcinomas,EBVaGC)和EBV阴性胃癌(EBV negative gastric carcinomas,EBVnGC)组织中c-Met基因的扩增及表达,探讨c-Met基因扩增和表达与EBV感染的相关性以及二者在胃癌发生发展中的作用.方法:应用半定量多聚酶链反应(PCR)检测EBVaGC 13例和EBVnGC 45例组织中c-Met基因的扩增,免疫组化抗原热修复技术检测c-Met蛋白表达.结果:EBVaGC和EBVnGC组织中均检测到c-Met基因扩增,EBVaGC组织与EBVnGC组织间c-Met基因的扩增水平无显著性差异.EBVaGC癌组织c-Met基因过度扩增率为53.8%(7/13),EBVnGC癌组织c-Met基因过度扩增率为44.4%(20/45),两组之间无差异.EBVaGC和EBVnGC癌组织中c-Met蛋白阳性率分别为76.9%(10/13)和64.4%(29/45),两组之间无显著性差异.EBVaGC和EBVnGC癌组织中c-Met蛋白过表达率分别为69.2%(9/13)和37.8%(17/45),统计学分析表明EBVaGC癌组织中c-Met蛋白的过表达高于EBVnGC组,两组之间有差异(χ2=4.0 345,P=0.0 446).EBVaGC和EBVnGC癌组织中c-Met基因的表达和扩增与肿瘤大小、肿瘤部位、淋巴结转移、分化程度以及临床分期等均无相关性.结论:胃癌组织中均存在c-Met基因的扩增,EBVaGC与EBVnGC组织中c-Met基因扩增水平和c-Met蛋白阳性率无明显差异,但EBVaGC癌组织中c-Met蛋白的过表达率高于EBVnGC组.
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