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姜黄素对溃疡性结肠炎小鼠肠黏膜内NF-κB及TNF-α表达的影响
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 2
- DOI:
- 10.3969/j.issn.1009-3079.2005.02.024
- Key Word:
- No keyword available
Abstract： 目的:观察姜黄素对小鼠溃疡性结肠炎的抗炎效果,探讨其作用机制.方法:选Balb/c小鼠40只,随机平均分为4组.A组乙醇灌肠为阴性组,D组噁唑酮(OXZ)灌肠为阳性组,C组在D组基础上按20 mg/kg的姜黄素处理,B组在D组基础上按40 mg/kg的姜黄素处理,3 d后进行炎症活动指数(DAI)、大体形态损伤、肠黏膜髓过氧化物酶(MPO)活性及组织学改变评分,再用免疫组化S-P法检测组织标本NF-κB、TNF-α的表达并评分.结果:A组DAI、大体形态、组织学损伤、MPO、NF-κB、TNF-α的评分依次是0.9±0.74、1.6±0.52、4.1±1.10、0.8±0.63、1.2±0.65、1.3±0.48;B组4.5±0.85、3.3±0.67、14.2±1.40、1.5±0.71、2.8±0.79、2.6±0.70;C组5.9±0.99、4.2±0.63、15.3±1.64、1.7±0.67、3.7±0.95、3.9±0.88:D组10.5±0.97、6.8±0.79、21.3±1.49、3.7±0.95、7.6±1.43、8.1±1.97.经姜黄素处理的炎症小鼠炎症结肠肠黏膜内NF-κB和TNF-α表达显著下降(P＜0.01),提示其结肠炎症明显减轻.NF-κB与TNF-α的表达显著相关(r=0.998,P＜0.01).结论:姜黄素是治疗溃疡性结肠炎的一种有效药物,其机制可能是通过抑制NF-κB的激活和TNF-α的表达而达到抗炎效果的.
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