Paper - WANFANG MED ONLINE

You Position: Home > Paper

Morphology of apoptosis of human hepatocellular carcinoma cell line induced by 2-[(3-carboxy-1-oxoprogy1) amino]-2-deoxy-D-glucose

( views:170, downloads:4 )

Author:: No author available

Journal Title:: WORLD CHINESE JOURNAL OF DIGESTOLOGY

Issue:: 9

DOI:: 10.3969/j.issn.1009-3079.2004.09.009

Key Word:: No keyword available

Abstract：目的:探讨2-(3-羧基-1-丙酰氨基)-2-脱氧-D-葡萄糖诱导肝癌细胞株HepG2凋亡的可能性.方法:选用不同浓度的2-(3-羧基-l-丙酰氨基)-2-脱氧-D-葡萄糖与肝癌细胞株共同孵育不同时间后,应用倒置相差显微镜、荧光显微镜、透射电子显微镜对细胞生长状况及药物作用后细胞的形态进行观察.结果:经2-(3-羧基-1-丙酰氨基)-2-脱氧-D-葡萄糖作用24-72 h后,HepG2肝癌细胞株出现体积缩小,荧光染色增强,胞核或胞质中可见致密浓染的块状或颗粒状黄绿色荧光染色.染色质固缩,并凝结成块,聚集在核膜周边呈新月状或肾状,胞质浓缩,内质网疏松并与胞膜融合形成-个个空泡.且凋亡细胞含量呈一定的浓度、时间相关性.结论:2-(3-羧基-1-丙酰氨基)-2-脱氧-D-葡萄糖具有诱导HepG2细胞凋亡的作用.

Reference

[1]Deng LY,Zhang YH,Xu P,etc.Expression of interleukin 1β converting enzyme in 5-FU induced apoptosis in esophageal carcinoma cells[J].1999,(1).
[2]李芳秋,武建国.凋亡相关基因突变与人类疾病[J].2002,(4).
[3]侯宝华,卿三华,董凤英,等.腹腔热灌注化疗对人大肠癌细胞裸鼠腹腔种植的影响[J].2000,(6).
[4]周培根,尤瑜敏,戚晓玉,等.氨基葡萄糖盐酸盐的制备及其某些性质[J].2000,(1).
[5]马吉祥,苏军英,马金姝,等.大豆异黄酮诱导食管癌细胞凋亡作用研究[J].2003,(11).
[6]杨连君,王文亮,司晓辉.乙醇诱导肝癌细胞凋亡的形态学观察[J].2001,(16).
[7]Bao Hua Sun,Xi Ping Zhao,Bao Ju Wang,etc.FADD and TRADD expression and apoptosis in primary hepatocellular carcinoma[J].2000,(2).
[8]Pei Lin Huang,Zhen Sheng Dai,Yue Lin Jin,etc.Inhibitor of fatty acid synthase induced apoptosis in human colonic cancer cells[J].2000,(2).
[9]李娟,单长民.肝癌中的细胞凋亡及其药物对它的诱发作用[J].2001,(4).
[10]邵红莲,辛华,高选,等.大蒜素诱导人肝癌BEL-7402细胞凋亡[J].2001,(3).
[11]吴勤,付体权,冯传前.茶多酚诱导肝癌细胞凋亡[J].2001,(2).
[12]袁淑兰,王艳萍,李良平,等.叶酸诱导人肝癌细胞凋亡及其机制的研究[J].2001,(4).
[13]王哲,乔岩,黄高升,等.氨基葡糖及其盐酸盐诱导白血病细胞K562向巨噬细胞样分化[J].2003,(3).
[14]刘剑仑,埃高莫·比佐,韦长元,等.康莱特注射液诱导人肝癌细胞凋亡的研究[J].2001,(4).
[15]司斌,刘志遐.诱导分化促进肺癌细胞对化疗药诱导凋亡的敏感性[J].2001,(4).
[16]王哲,乔岩,黄高昇,等.N-乙酰氨基葡萄糖诱导白血病细胞K562向巨噬细胞分化[J].2003,(1).
[17]朴云峰,时阳,高普均.肝癌细胞的诱导分化及凋亡的研究[J].2001,(4).
[18]张倜,王绍山,齐清会.砷剂作用下大鼠肝癌的形态学及细胞动力学变化[J].2001,(2).
[19]谢秋玲,戴云,孙奋勇,等.盐酸氨基葡萄糖胺诱导黑素瘤细胞分化的形态学观察[J].2002,(3).
[20]罗俊卿,闻勤生,焦成松.三氧化二砷诱导肝癌HepG2细胞凋亡作用[J].2001,(1).
[21].Antisense Telomerase Rna Induced Human Gastric Cancer Cell Apoptosis[J].2000,(3).
[22]Naoki Hino,Toshihiro Higashi,Kazuhiro Nouso,etc.Apoptosis and proliferation of human hepatocellular carcinoma[J].1996,16(2).
[23]J F, Kerr,C M, Winterford,B V, Harmon.Apoptosis. Its significance in cancer and cancer therapy.[J].1994,73(8).
[24]K M, Eck,L, Yuan,L, Duffy,etc.A sequential treatment regimen with melatonin and all-trans retinoic acid induces apoptosis in MCF-7 tumour cells.[J].1998,77.
[25].A Study on Arsenic Trioxide Inducing in Vitro Apoptosis of Gastric Cancer Cell Lines[J].2000,(3).
[26]KONG Xiang-ping,ZOU Qing-yan,LI Ru-bing,etc.Apoptosis of neoplasm cell lines induced by hepatic peptides extracted from sucking porcine hepatocytes[J].1999,(5).
[27]Kerr J F R,Wyllie A H,Currie A R.Apoptosis: A Basic Biological Phenomenon with Wideranging Implications in Tissue Kinetics[J].1972.
[28].Early Apoptosis in Intestinal and Diffuse Gastric Carcinomas[J].2000,(6).
[29]Daniel R. Catchpoole,Bernard W. Stewart.Formation of Apoptotic Bodies Is Associated with Internucleosomal DNA Fragmentation during Drug-Induced Apoptosis[J].1995,216(1).