You Position: Home > Paper

Cloning and expression of gastric cancer related gene GCRG224 in E.coli

( views:0, downloads:0 )
Author:
No author available
Journal Title:
WORLD CHINESE JOURNAL OF DIGESTOLOGY
Issue:
4
DOI:
10.3969/j.issn.1009-3079.2004.04.002
Key Word:
No keyword available

Abstract´╝Ü 目的:利用硫氧还蛋白融合表达系统表达胃癌相关基因GCRG224.方法:采用PCR技术从pGEM-T质粒上扩增出含完整ORF的GCRG224 cDNA序列,将其克隆至硫氧还蛋白融合表达载体pET102/D-TOPO中,转化大肠杆菌BL21,经IPTG诱导表达重组融合蛋白,SDS-PAGE分析表达产物.结果:高效表达出相对分子量约16 800的重组融合蛋白.薄层凝胶扫描显示,其表达量占菌体总蛋白质的22.3%.结论:在大肠杆菌中成功表达了GCRG224重组融合蛋白.

WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615 Email:yiyao@wanfangdata.com.cn