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Cloning and expression of gastric cancer related gene GCRG224 in E.coli
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 4
- DOI:
- 10.3969/j.issn.1009-3079.2004.04.002
- Key Word:
- No keyword available
Abstract： 目的:利用硫氧还蛋白融合表达系统表达胃癌相关基因GCRG224.方法:采用PCR技术从pGEM-T质粒上扩增出含完整ORF的GCRG224 cDNA序列,将其克隆至硫氧还蛋白融合表达载体pET102/D-TOPO中,转化大肠杆菌BL21,经IPTG诱导表达重组融合蛋白,SDS-PAGE分析表达产物.结果:高效表达出相对分子量约16 800的重组融合蛋白.薄层凝胶扫描显示,其表达量占菌体总蛋白质的22.3%.结论:在大肠杆菌中成功表达了GCRG224重组融合蛋白.
- [1]Gang-Shi Wang,Meng-Wei Wang,Ben-Yan Wu,etc.A novel gene, GCRG224, is differentially expressed in human gastric mucosa[J].2003,(1).
- [2]Yun ZHOU,Shan-Shan Gao,Yong-Xin Li,etc.Tumor suppressor gene p16 and Rb expression in gastric cardia precancerouslesions from subjects at a high incidence area in northern China[J].2002,(3).
- [3]王孟薇,杨少波,张子其,等.老年人胃癌前黏膜癌变的胃镜随访[J].2003,(9).
- [4]郝冬梅,孙秀菊,郑志红,等.胃癌癌前病变相关基因的筛查及表达研究[J].2003,(1).
- [5]吴云林,陈颖.早期胃癌的临床筛选研究[J].2002,(12).
- [6]Jian-Bang Lu,Xi-Bin Sun,Di-Xin Dai,etc.Epidemiology of gastroenterologic cancer in Henan Province, China[J].2003,(11).
- [7]黄海力,吴本俨,尤纬缔,等.进展期胃癌病理和预后影响因素的关系[J].2003,(9).
- [8]朱慧,刘伟,史蔚,等.重组人尿激酶原的体外变复性研究[J].2001,(2).
- [9]王刚石,王孟薇,尤纬缔,等.人胃癌低表达cDNA片段的克隆与初步表达分析[J].2001,(1).
- [10]潘传敬,刘宽宇.胃癌增生凋亡与调节基因的表达[J].2003,(5).
- [11]J J, Chalmers,E, Kim,J N, Telford,etc.Effects of temperature on Escherichia coli overproducing beta-lactamase or human epidermal growth factor.[J].1990,56.
- [12]Y, Hattori,H, Itoh,S, Uchino,etc.Immunohistochemical detection of K-sam protein in stomach cancer.[J].1996,2.
- [13]Laurence, Van Melderen.Molecular interactions of the CcdB poison with its bacterial target, the DNA gyrase.[J].2002,291(6-7).
- [14]H, Yokozaki,W, Yasui,E, Tahara.Genetic and epigenetic changes in stomach cancer.[J].2001,204.
- [15]Amemiya H,Kono K,Takahashi A,etc.c-Met Expression in Gastric Cancer with Liver Metastasis.[J].2002,63(3).
- [16]Jinyoung, Yoo,Sonya Y, Park,Robert A, Robinson,etc.ras Gene mutations and expression of Ras signal transduction mediators in gastric adenocarcinomas.[J].2002,126.
- [17]Anton P J, Middelberg.Preparative protein refolding.[J].2002,20(10).
- [18]Boussioutas A,Taupin D.Towards a molecular approach to gastric cancer management.[J].2001,31(5).
- [19]Ebihara Y,Ishii HH,Gobe GC,etc.Apoptosis and cell proliferation in the development of gastric carcinomas: Associations with c-myc and p53 protein expression.[J].2002,17(9).
- [20]D C, Fang,Y H, Luo,R, Lu,etc.Studies on the relationship between the point mutation of ras oncogenes and the prognosis of patients with gastric cancer.[J].1997,3.
- [21]McKenna BJ,Ross JS.The HER-2/neu oncogene in tumors of the gastrointestinal tract.[J].2001,19(5).
- [22]Wyns L,Dao Thi MH,Maes D,etc.Intricate interactions within the ccd plasmid addiction system.[J].2002,277(5).
- [23]Mai M,Yamashita K,Takahashi Y,etc.Abnormal expression of E-cadherin, beta-catenin, and c-erbB-2 in advanced gastric cancer: its association with liver metastasis.[J].2003,18(2).
- [24]Becker KF,Keller G,Hoefler H.The use of molecular biology in diagnosis and prognosis of gastric cancer.[J].2000,9(1).
- [25]Patrick Gavit.Production of antifungal recombinant peptides in Escherichia coli[J].2000,79(2).