Effects of hyperbaric oxygen with anti-free radicals on expression of matrix metalloproteinase-2 in rat liver
- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 12
- DOI:
- 10.3969/j.issn.1009-3079.2003.12.013
- Key Word:
- No keyword available
Abstract： 目的:观察高压氧加自由基拮抗剂对大鼠肝脏损伤早期及肝纤维化形成过程中肝脏基质金属蛋白酶-2(MMP-2)表达的影响方法:用CCl4建立大鼠肝纤维化模型,在2 wk和8 wk分别给予高压氧结合自由基拮抗剂、高压氧、自由基拮抗剂处理,放免法测定血清透明质酸(HA)的含量,以了解细胞外基质合成情况,免疫组化法检测肝组织MMP-2蛋白以及其调节相关的生长因子TGF-β1蛋白的表达;应用逆转录-聚合酶链反应(RT-PCR)方法检测MMP-2 mRNA水平.结果:实验2 wk末模型组高压氧结合自由基拮抗剂组肝脂肪变性和水样变性及点状坏死与模型组相比明显减轻,MMP-2蛋白及mRNA,TGF-β1蛋白表达均低于模型组,差异显著(MMP-2:5.04±1.34 vs 56.75±3.03,P＜0.01;MMP-2 mRNA:0.155±0.005 vs 0.547±0.013,P＜0.01;TGF-β1:3.28±0.33 vs 4.96±0.28,P＜0.01).血清HA虽低于模型组,但无显著差异(P＞0.05).实验8 wk末模型组肝小叶内纤维结缔组织增生明显并形成粗细不等的纤维条索,高压氧结合自由基拮抗剂组以肝细胞脂变和细胞水肿为主,纤维结缔组织无明显增生,肝组织MMP-2蛋白及mRNA,TGF-β1蛋白表达和血清HA水平与模型组相比均明显为低(MMP-2:12.53±2.51 vs 160.57±5.54,P＜0.01;MMP-2 mRNA:0.506±0.013 vs 0.685±0.014,P＜0.01;TGF-β1:4.97±0.35 vs 7.76±0.39,P＜0.01;HA:116.8±17.7μg/L vs 87.8±31.6μg/LP＜0.01).高压氧组、自由基拮抗剂组在2 wk末和8 wk末肝损伤略轻于模型组,MMP-2蛋白和TGF-β1蛋白表达均低于模型组,且高压氧组MMP-2 mRNA表达亦较模型组低.结论:高压氧结合自由基拮抗剂可以降低CCl4损伤大鼠肝脏MMP-2表达,可以迟滞肝纤维化病变的发展.
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