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端粒酶在大肠癌细胞中的活性表达及临床意义
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 10
- DOI:
- 10.3969/j.issn.1009-3079.2003.10.045
- Key Word:
- No keyword available
Abstract： 目的:探讨端粒酶活性的改变在大肠癌发生发展过程中的作用以及端粒酶活性检测作为一种新的大肠癌临床诊断的生物学标志的可行性.方法:采用TRAP(telomeric repeat amplification protocol)结合聚酰胺凝胶电泳银染法检测46例配对大肠癌及癌旁组织,4例大肠癌肝转移病灶组织内端粒酶的活性,并用Southem-blot-ECL(enhanced chemiluminescecce)法对上述标本内端粒酶平均长度进行检测.结果:肿瘤组内端粒酶活性明显高于对应癌旁组织,在肿瘤组织内,端粒酶的活性表达升高与大肠癌的病理学分期呈显著相关性(P＜0.01,t=8.3477),而与肿瘤的大小、部位,肿瘤的恶性程度、分级、浸润深度、CEA表达水平等无显著相关性,与肿瘤组相比,在肝转移病灶内,端粒酶的活性表达无显著升高,肿瘤组内的端粒酶平均长度明显大于对应的癌旁组织.端粒酶平均长度与大肠癌的病理学分期呈显著相关性.结论:端粒酶的活化及其长度的缩短可能成为早期检测大肠癌癌变的临床指标.
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