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Lymphatic corrosion casts in rabbit ileum: scanning electronmicroscopic studies

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Author:: No author available

Journal Title:: WORLD CHINESE JOURNAL OF DIGESTOLOGY

Issue:: 4

DOI:: 10.3969/j.issn.1009-3079.2003.04.018

Key Word:: No keyword available

Abstract：目的:观察家兔回肠淋巴管的三维结构和微细分布.方法:淋巴管铸型样品,通过扫描电子显微镜进行观察;半薄切片样品,用光镜进行观察.淋巴管铸型剂是Mercox,采用回肠壁间接注射法.将注入铸型剂的回肠壁切下,置入NaOH水溶液中腐蚀,直到肠壁组织完全脱离为止,然后将淋巴管铸型样品置于扫描电子显微镜下观察.结果:在小肠绒毛内清晰地显示出中央乳糜管,每个绒毛中有2-3个中央乳糜管.中央乳糜管与黏膜层毛细淋巴管丛相连通.黏膜层毛细淋巴管注入黏膜下层淋巴管,后者与肌层淋巴管相吻合.肌层淋巴管连接浆膜层淋巴管,然后汇入小肠系膜淋巴管.淋巴管呈串珠样外观,其表面存有双凹切迹,该处相当于淋巴瓣的部位.铸型表面还可以见到淋巴管内皮细胞核的压迹.结论:淋巴管铸型清晰地显示家兔回肠壁黏膜层丰富的中央乳糜管和毛细淋巴管丛以及黏膜下层、肌层毛细淋巴管和大量的淋巴管的三维结构.

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