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Effect of arsenic trioxide on expression of telomerase and induction of apoptosis of human hepatoma cell
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 3
- DOI:
- 10.3969/j.issn.1009-3079.2003.03.004
- Key Word:
- No keyword available
Abstract： 目的:观察三氧化二砷对体外培养的人肝癌细胞株细胞端粒酶表达和细胞生长抑制的影响及诱导细胞凋亡的作用,探讨三氧化二砷的抗肿瘤作用及机制.方法:采用噻唑蓝比色分析法(MTT法),流式细胞分析,原位杂交方法以及TUNEL原位末端标记法观察和检测了不同浓度的三氧化二砷对人肝癌细胞株(SMMC-7721)细胞的增生,细胞DNA含量的分布,细胞端粒酶表达的影响及细胞凋亡的诱导作用.结果:20,5,5 μmol/L三氧化二砷在24,48,72 h时抑制率分别为22.0%,25.7%,32.0%,均能明显抑制人肝癌细胞株细胞的增生(单侧置信区间为10.1,23.7,18.0,P＜0.05).流式细胞分析显示加药组G0/G1期细胞比例减少,S期细胞所占比例增高.随着药物浓度的增加细胞端粒酶的表达下降,而凋亡细胞数增加.结论:三氧化二砷能抑制人肝癌细胞增生,其抑制作用具有时间-计量效应关系,并能抑制肝癌细胞端粒酶的表达及诱导肝癌细胞凋亡.
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