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Detection of different viral transcripts in sera of patients with chronic hepatitis B virus infection

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Author:: No author available

Journal Title:: WORLD CHINESE JOURNAL OF DIGESTOLOGY

Issue:: 2

DOI:: 10.3969/j.issn.1009-3079.2003.02.003

Key Word:: No keyword available

Abstract：目的:建立针对乙型肝炎病毒(HBV)全长型(fRNA)和顿挫型转录体(trRNA)的血清学检测方法,并探讨其临床应用价值.方法:自患者血清中提取病毒核酸,经PCR及PRT-PCR进行扩增,琼脂糖电泳显示产物,Southem印迹验证反应的特异性.取代表性产物克隆,测序.结果:在HBV慢性携带者的血清中检测到了在上述不同poly(A)位点成熟的病毒RNA.血清中fRNA的检出与e抗原阳性和较强的病毒DNA信号相对应,与转氨酶活性呈正相关,这一血清学指标反映了病毒复制;而trRNA的检出与e抗原阳性和转氨酶活性无关,不象fRNA那样依赖于血清中病毒DNA的量.二者检出率随年龄有不同变化,前者随年龄增加而进行性减低乃至转阴;而后者在10-20岁期间检出率升至80%,以后维持在较高水平.即使在某些表面抗原阴性血清中,也能检测到trRNA和不在上述两个位点成熟的病毒RNA.这些转录体在某些隐源性肝硬变和丙型肝炎患者血清中也存在.结论:病毒转录体的血清学检测有助于确定目前尚不认识的HBV感染期,可用来更准确地区分复制期和非复制期以及检出隐匿性HBV感染.

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