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乙型肝炎病毒核心基因启动子序列突变及准种
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 11
- DOI:
- 10.3969/j.issn.1009-3079.2001.11.030
- Key Word:
- 肝炎病毒,乙型/遗传学;基因,病毒/遗传学;启动区(遗传学);突变
Abstract： 目的通过对乙型肝炎病毒(HBV)基本核心基因启动子(BCP)变异的研究,阐明HBv准种在慢性感染者中存在的意义 .方法以中国株HBV基因序列为依据,设计特异性多聚酶链反应(PCR)引物,自43例慢性HBV感染患者血清中扩增HBV的BCP基因,采用聚丙烯酰胺凝胶电泳(PAGF)技术展示缺失突变,DNA测序确定病毒的变异程度.结果聚丙烯酰胺凝胶电泳结果发现,57.8%患者血清中可见2-3条扩增条带;测序结果发现BCP区存在多种突变形式,点突变中以140nt(T→C)最为常见,缺失突变多见于TA1,TA2及TA3,多表现为8bp、20bp的缺失.结论HBV BCP区内有一缺失高变区,并在患者个体体内存有多种变异形式;TAl,TA2及TA3的变异可能影响e抗原的表达.结果提示HBv慢性携带者体内有HBv准种共存
- [1]成军.乙型肝炎病毒准种研究的意义[J].2001,(4).
- [2]黄耀煊,邬光惠.乙型肝炎与肝癌关系[J].2000,(z1).
- [3]董菁,成军,王勤环,等.慢性乙型肝炎患者体内乙型肝炎病毒准种特点的初步研究[J].2001,(4).
- [4]Michael Friedt,Patrick Gerner,Ekkehart Lausch,etc.Mutations in the basic core promotor and the precore region of hepatitis b virus and their selection in children with fulminant and chronic hepatitis B[J].1999,29(4).
- [5]Lee, Young Ik,Lee, Sook,Lee, Yoonik,etc.The human hepatitis B virus transactivator X gene product regulates Sp1 mediated transcription of an insulin-like growth factor II promoter 4.[J].1998,16(18).
- [6]Gustavson C,Dhillon AP,Mardberg K,etc.Mutation of nucleotide 1,762 in the core promoter region during hepatitis B e seroconversion and its relation to liver damage in hepatitis B e antigen carriers.[J].1998,55(3).
- [7]Kew MC,Kramvis A.The core promoter of hepatitis B virus.[J].1999,6(6).
- [8]Kojima N,Onji M,Horiike N,etc.In situ detection of mutated hepatitis B virus in microdissected, formalin-fixed liver tissues from patients with chronic hepatitis B.[J].1999,30(3).
- [9]Piwon N,Will H,Gunther S.Wild-type levels of pregenomic RNA and replication but reduced pre-C RNA and e-antigen synthesis of hepatitis B virus with C(1653) --> T, A(1762) --> T and G(1764) --> A mutations in the core promoter.[J].1998,79(2).
- [10]王小众,陶其敏.乙肝病毒X基因与肝癌[J].1999,(12).
- [11]Tratzmuller R,Wirth S,Friedt M,etc.Hepatitis B virus core promoter mutations in children with multiple anti-HBe/HBeAg reactivations result in enhanced promoter activity.[J].1999,59(4).
- [12]Marrone A,Liang TJ,Baumert TF,etc.Naturally occurring mutations define a novel function of the hepatitis B virus core promoter in core protein expression.[J].1998,72(8).
- [13]Nishizono A,Kohno K,Hiraga M,etc.Reduced transcription and progeny virus production of hepatitis B virus containing an 8-bp deletion in basic core promoter.[J].2000,61(1).
- [14]Lee SG,Rho HM.Transcriptional repression of the human p53 gene by hepatitis B viral X protein.[J].2000,19(3).