Association between lowe expression of somatostatin receptor Ⅱ gene and lymphoid metastasis in patients with gastric cancer
- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 10
- DOI:
- 10.3969/j.issn.1009-3079.1999.10.010
- Key Word:
- 胃肿瘤;受体,生长抑素;基因表达;淋巴转移
Abstract： 目的 恶性肿瘤的发生和转移机制尚未完全明确,我们旨在研究胃癌癌灶(C)、癌旁组织(P)和远处正常组织(N)生长抑素(SS)含量及其Ⅱ型受体(SSRⅡ)基因的表达情况,及其与腹腔淋巴结转移的关系,探讨SS及SSRⅡ在胃癌发生、发展和转移中的作用.方法 外科手术切除的胃癌标本36例,分为2组:腹腔淋巴结转移组(A组)20例,男17例,女3例,年龄58.4岁±14.8岁;B组(无淋巴结转移)16例,男14例,女2例,年龄60,5岁±14.2岁.大体标本离体后立即取癌灶、癌旁和远处正常组织,应用RIA法检测其SS含量、同位素掺入RT-PCR法检测SSRⅡ基因表达情况(以cpm高低间接反映基因的表达强弱).结果 A,B两组的年龄和性别分布无显著性差异(P＞0.05).癌灶SS含量明显低于癌旁和正常粘膜(P＜0.05),但A,B两组间的差异无统计学差异(pmol/g,A组:C 15±8,P 30±8,N 32±11;B组:C 16±9,P 32±10,N 36±12).A组癌灶SSRⅡ基因表达明显低于癌旁和远处粘膜(P＜0.01),也低于B组癌灶的SSRⅡ的表达(P＜0.01),但B组癌灶、癌旁组织和远处粘膜组织RNA中SSRⅡ基因的表达相似(min-1@μg-1,A组:C 1835±824,P 3458±1025,N 3379±967;B组:C 2996±1014,P 3347±1117,N 3245±1047).结论 上述结果表明胃癌(不论有无腹腔淋巴结转移)癌灶中SS含量明显降低,但仅有腹腔淋巴结转移的胃癌癌灶SSRⅡ基因表达低下,提示SS在胃癌的发生发展过程中起一定的作用,而Ⅱ型SS受体表达减少可能与胃癌的转移有关.
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