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Construction of retroviral vector containing HSV-tk gene for colorectal carcinoma tissue-specific gene therapy

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Author:
No author available
Journal Title:
WORLD CHINESE JOURNAL OF DIGESTION
Issue:
8
DOI:
10.3969/j.issn.1009-3079.1998.08.002
Key Word:
herpes simplex virus;thymidine kinase gene;colorectal carcinoma;transcriptional regulatory sequences

Abstract: AIM In order to express suicide gene specifically in colorectal carcinoma cells for targeted gene therapy, we constructed the recombinant retroviral vector promoted by transcriptional regulatory sequences of carcinoembryonic antigen (CEA) gene.METHODS A 1.3 kb EcoRⅠ/ BamHⅠ fragment of the HSV-tk gene was isolated from pLNtk and inserted into pUC118, then the HindⅢ/ BamHⅠ fragment from pCEA424/ 2CAT was isolated and subcloned into pUC118 to harvest HindⅢ/ EcoRⅠ CEA TRS. These two fragments were ligated with the retroviral vector GINa.RESULTS Restriction enzyme Sal Ⅰwas used to identify a positive clone B5. The recombinant retroviral vector, GICEAtkNa was constructed.CONCLUSION Success in construction of GICEAtkNa provides a basis for colorectal carcinoma-specific gene therapy.

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