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Cordycepin induces apoptosis of cutaneous T-cell lymphoma Hut78 cells via mitochondrial pathway

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Author:
No author available
Journal Title:
International Journal of Dermatology and Venereology
Issue:
3
DOI:
10.3760/cma.j.issn.2096-5540.2018.03.006
Key Word:
Cordycepin;Hut78 cell line;Apoptosis;Proliferation

Abstract: Objective Cordycepin is the active component of Cordyceps sinensis and Cordyceps militaris,belongs to Traditional Chinese Medicine,and has a significant anti-cancer activity.This research aimed to study the roles of cordycepin in inducing apoptosis of cutaneous T-cell lymphoma Hut78 cell line.Methods After treatment with 5 μg/ml cordycepin for 24 hours,the proliferation and apoptosis of Hut78 cells induced by cordycepin were analyzed via MTT and flow cytometry assay,respectively,qRT-PCR and Western blotting were used to investigate the levels of apoptosis-related proteins.Results Proliferation and apoptosis of Hut78 cells were inhibited by cordycepin in a dose-dependent manner.The expression levels of apoptosis-related proteins including caspase-8,cleaved caspase-3,tBid,Bax,Apafl,AIF and DR3 receptor were all decreased in the cells stimulated with 5 μg/ml cordycepin for 24 hours compared with those in the control group.Conclusion Cell proliferation and induced apoptosis could be inhibited by cordycepin via the DR3/mitochondrial signaling pathway.

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