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聚合酶链反应检测沙眼衣原体的研究进展
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- Author:
- No author available
- Journal Title:
- INTERNATIONAL JOURNAL OF DERMATOLOGY AND VENEREOLOGY
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.1673-4173.2002.03.012
- Key Word:
- 衣原体,沙眼;聚合酶链反应
Abstract： 自从1993年正式商业化以来,聚合酶链反应检测沙眼衣原体的许多方面有了长足的进展.检测标本除了拭子、尿液,还增加了棉塞标本.邮寄自取标本的应用有望提高沙眼衣原体筛查的普及率.已经明确许多原因可导致聚合酶链反应抑制,另一方面,对标本冻融、保存,稀释、提纯及加入特殊物质等均可不同程度地降低聚合酶链反应抑制率.内参照用于监测聚合酶链反应抑制情况,进一步提高了聚合酶链反应检测的敏感性.β-球蛋白基因扩增可以实现对标本质量的评价.聚合酶链反应方法中新出现了TETR-聚合酶链反应,Q-PNA聚合酶链反应,混合标本聚合酶链反应及自动化聚合酶链反应等.
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