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Role of MHC class Ⅱ transactivator in upregulating the expression of HLA class Ⅱ antigen in HepG2 cells
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- Author:
- No author available
- Journal Title:
- ACTA ACADEMIAE MEDICINAE MILITARIS TERTIAE
- Issue:
- 21
- DOI:
- 10.3321/j.issn:1000-5404.2007.21.001
- Key Word:
- MHCⅡ类反式激活因子;HepG2细胞;HLAⅡ类分子
Abstract： 目的 探讨主要组织相容性复合物(major histocompatibility complex,MHC)Ⅱ类反式激活因子(class Ⅱ transactivator , CⅡTA)在调控HepG2细胞HLAⅡ类分子表达中的作用.方法 将含CⅡTA cDNA的真核表达载体EBS-NPL-CⅡTA和不含CⅡTA cDNA的空载体EBS-NPL分别转染至HepG2细胞.用RT-PCR技术检测未经转染的HepG2细胞、转染EBS-NPL-CⅡTA及空载体EBS-NPL的HepG2细胞CⅡTA mRNA的表达,并用间接细胞免疫荧光技术及流式细胞技术检测其3种HLAⅡ类分子(HLA-DR、DP、DQ)的表达.结果 未经转染的HepG2细胞和转染空载体EBS-NPL的HepG2细胞均无CⅡTA mRNA和HLAⅡ类分子的表达.转染EBS-NPL-CⅡTA后的HepG2细胞出现CⅡTA mRNA表达,并表达3种HLAⅡ类分子.结论 CⅡTA是调控HepG2细胞是否表达HLAⅡ类分子的关键因子,HepG2细胞不表达HLA-Ⅱ类分子与其缺乏CⅡTA表达有关,为进一步研究CⅡTA在肝癌治疗中的作用奠定基础.
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