Casticin inhibits proliferation and invasion of breast cancer MCF-7 cells

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Author:
HUANG Ji-hong(TCM Pharmacy,Cancer Hospital of Shantou University Medical College,Shantou 515041,China)
CHEN Shao-ying()
ZHENG Jian()
Journal Title:
Cancer Research and Clinic
Issue:
Volume 24, Issue 08, 2012
DOI:
10.3760/cma.j.issn.1006-9801.2012.08.007
Key Word:
Casticin; Breast neoplasms; Cell proliferation; Neoplasm invasiveness

Abstract: Objective To investigate the effects of casticin on proliferation and invasion on human breast cancer MCF-7 cells.Methods MTT assay and invasion assay were performed in MCF-7 cells treated with casticin,respectively.The expression of Bax,Caspase-3,MMP-2 and MMP-9 was analyzed by RT-PCR and Western blot.Results Casticin inhibited MCF-7 cells proliferation and invasion in a dose-dependent manner (P < 0.05).Additionally,Bax and Caspase-3 expression was increased in MCF-7 cells treated with casticin,whereas MMP-2 and MMP-9 were down-regulated.Conclusion Casticin may play a critical role in inhibiting breast tumor cell proliferation and invasion,reinforcing its potential in clinical application.

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